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Amplite® Fluorimetric DPP4 Activity Assay Kit
The Amplite® Fluorimetric DPP4 Activity Assay Kit provides a simple, quick, and direct protocol for measuring DPP4 Activity. This assay leverages the DPP4-mediated cleavage of a non-fluorescent substrate, resulting in a fluorescent product with Ex/Em = 360/460 nm, respectively. The fluorescence intensity generated is directly proportional to the DPP4 activity in the sample, making it suitable for detecting DPP4 in various biological matrices such as cellular lysates, tissue extracts, and serum. Additionally, the assay is compatible with high-throughput screening systems. One unit (U) is the amount of enzyme that catalyzes the reaction of 1 µmol of substrate per minute. Dipeptidyl peptidase-4, also known as DPP4, CD26, ADCP2, DPP, is a transmembrane glycoprotein belonging to the prolyl oligopeptidase family. It is a serine exopeptidase that cleaves X-proline and X-Alanine residues from the N-terminal ends of polypeptides. DPP4 is involved in several physiological processes, including glucose metabolism through the regulation of glucagon-like-peptide (GLP-1), immune regulation by acting as a receptor on many immune cells, signal transduction as a transmembrane protein responsive to growth factors and chemokines, and tumor suppression via immune modulation. DPP4 inhibitors are being used as a treatment for type-2 diabetes.
AMC dose response was measured with the Amplite® Fluorimetric DPP4 Activity Assay Kit on a 96-well black microplate after incubation for 30 min at room temperature. The fluorescence intensity was monitored using a Gemini fluorescence microplate reader (Molecular Devices) Ex/Em = 360/460 nm, Cutoff = 435 nm.
AMC dose response was measured with the Amplite® Fluorimetric DPP4 Activity Assay Kit on a 96-well black microplate after incubation for 30 min at room temperature. The fluorescence intensity was monitored using a Gemini fluorescence microplate reader (Molecular Devices) Ex/Em = 360/460 nm, Cutoff = 435 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11323100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation360 nm
Emission460 nm
Cutoff435 nm
Recommended plateSolid black or black plate with clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on December 7, 2025