Name: Amplite® Fluorimetric DPP4 Activity Assay Kit
Brand: AAT Bioquest
SKU: 11323
Price: 516 USD
Availability: InStock

Amplite® Fluorimetric DPP4 Activity Assay Kit

Amplite® Fluorimetric DPP4 Activity Assay Kit is a fluorimetric assay kit for quantification of DPP4 activity in cellular lysates, tissue extracts, and serum.

Detection Principle: Detects DPP4-mediated cleavage of a non-fluorescent substrate.
Signal Response: Fluorescence intensity is proportional to DPP4 activity.
Instrument Compatibility: Fluorescence detection at Ex/Em 360/460 nm.
Workflow: Compatible with high-throughput screening systems.

AMC dose response was measured with the Amplite® Fluorimetric DPP4 Activity Assay Kit on a 96-well black microplate after incubation for 30 min at room temperature. The fluorescence intensity was monitored using a Gemini fluorescence microplate reader (Molecular Devices) Ex/Em = 360/460 nm, Cutoff = 435 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11323	100 Tests	Price

Overview

The Amplite® Fluorimetric DPP4 Activity Assay Kit provides a simple, quick, and direct protocol for measuring DPP4 Activity. This assay leverages the DPP4-mediated cleavage of a non-fluorescent substrate, resulting in a fluorescent product with Ex/Em = 360/460 nm, respectively. The fluorescence intensity generated is directly proportional to the DPP4 activity in the sample, making it suitable for detecting DPP4 in various biological matrices such as cellular lysates, tissue extracts, and serum. Additionally, the assay is compatible with high-throughput screening systems. One unit (U) is the amount of enzyme that catalyzes the reaction of 1 µmol of substrate per minute. Dipeptidyl peptidase-4, also known as DPP4, CD26, ADCP2, DPP, is a transmembrane glycoprotein belonging to the prolyl oligopeptidase family. It is a serine exopeptidase that cleaves X-proline and X-Alanine residues from the N-terminal ends of polypeptides. DPP4 is involved in several physiological processes, including glucose metabolism through the regulation of glucagon-like-peptide (GLP-1), immune regulation by acting as a receptor on many immune cells, signal transduction as a transmembrane protein responsive to growth factors and chemokines, and tumor suppression via immune modulation. DPP4 inhibitors are being used as a treatment for type-2 diabetes.

References

View all 50 references: Citation Explorer

Dipeptidyl-peptidase 4 (DPP4) mediates fatty acid uptake inhibition by glucose via TAS1R3 and GLUT-2 in Caco-2 enterocytes.

Authors:

Preinfalk, Verena and Kimmeswenger, Isabella and Somoza, Veronika and Lieder, Barbara

Journal:

Heliyon (2024): e30329

Diabetic individuals with COVID-19 exhibit reduced efficacy of gliptins in inhibiting dipeptidyl peptidase 4 (DPP4). A suggested explanation for increased COVID-19 susceptibility in patients with type 2 diabetes mellitus (T2DM).

Authors:

Mora-Rodríguez, José María and Sánchez, Belén G and Bort, Alicia and Díaz-Yuste, Alba and Ballester-González, Rubén and Arrieta, Francisco and Sebastián-Martín, Alba and Díaz-Laviada, Inés

Journal:

Life sciences (2024): 122292

Increased Thyroid DPP4 Expression Is Associated With Inflammatory Process in Patients With Hashimoto Thyroiditis.

Authors:

Wen, Xiaohui and Chang, Xiaona and He, Xueqing and Cai, Qingyun and Wang, Guang and Liu, Jia

Journal:

The Journal of clinical endocrinology and metabolism (2024): 1517-1525

Bidirectional relation between dipeptidyl peptidase 4 and angiotensin II type I receptor signaling.

Authors:

Martins, Flavia L and Ribeiro-Silva, Joao Carlos and Nistala, Ravi and Girardi, Adriana C C

Journal:

American journal of physiology. Cell physiology (2024): C1203-C1211

Pyrano[2,3-c]pyrazole fused spirooxindole-linked 1,2,3-triazoles as antioxidant agents: Exploring their utility in the development of antidiabetic drugs via inhibition of α-amylase and DPP4 activity.

Authors:

Chahal, Sandhya and Rani, Payal and Shweta, and Goel, Kapil Kumar and Joshi, Gaurav and Singh, Rajvir and Kumar, Parvin and Singh, Devender and Sindhu, Jayant

Journal:

Bioorganic chemistry (2024): 107363

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	360 nm
Emission	460 nm
Cutoff	435 nm
Recommended plate	Solid black or black plate with clear bottom

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Page updated on October 26, 2026

Component A: DPP4 Assay Buffer	25 mL
Component B: DPP4 Substrate	0.25 mL
Component C: DPP4 Positive Control	20 µL
Component D: AMC Standard	0.1 mL
Component E: DPP4 Inhibitor (Sitagliptin)	50 µL