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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors. DPP4 activity is quantified by the enzymatic cleavage of a specific substrate, resulting in a fluorescent product (Ex/Em = 360/460 nm), which correlates directly with the enzymatic activity present. This kit includes Sitagliptin, a well-characterized DPP4 inhibitor used in diabetes treatment, as a reference control, facilitating the assessment of inhibitor efficacy. The assay is optimized for high-throughput screening applications. Dipeptidyl peptidase-4 (DPP4), also referred to as CD26, ADCP2, or DPP, is a transmembrane glycoprotein within the prolyl oligopeptidase family. DPP4 functions as a serine exopeptidase, cleaving N-terminal X-proline and X-alanine residues from polypeptides. It is implicated in numerous physiological processes, including glucose metabolism via regulation of glucagon-like peptide-1 (GLP-1), immune modulation through its role as a receptor on various immune cells, signal transduction as a transmembrane protein responsive to growth factors and chemokines, and tumor suppression via immune system interactions. Thus, DPP4 inhibitors are critical in the therapeutic management of type-2 diabetes.
Sitagliptin dose response was measured with the Amplite® Fluorimetric DPP4 Inhibitor Screening Kit on a 96-well black microplate after incubation for 30 minutes at room temperature. The fluorescence intensity was monitored using a Gemini fluorescence microplate reader (Molecular Devices) Ex/Em = 360/460 nm, Cutoff = 435 nm.
Sitagliptin dose response was measured with the Amplite® Fluorimetric DPP4 Inhibitor Screening Kit on a 96-well black microplate after incubation for 30 minutes at room temperature. The fluorescence intensity was monitored using a Gemini fluorescence microplate reader (Molecular Devices) Ex/Em = 360/460 nm, Cutoff = 435 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11324100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation360 nm
Emission460 nm
Cutoff435 nm
Recommended plateSolid black or black plate with clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on December 25, 2025