Name: Amplite® Fluorimetric Endotoxin Detection Kit
Brand: AAT Bioquest
SKU: 60006
Price: 371 USD
Availability: InStock

Amplite® Fluorimetric Endotoxin Detection Kit

Amplite® Fluorimetric Endotoxin Detection Kit is a fluorimetric assay kit for detection of endotoxin in biological materials such as protein, peptide, and antibody samples.

Detection Principle: Uses LAL-mediated hydrolysis of a fluorogenic substrate.
Sensitivity: Detects endotoxin down to 0.001 EU/mL within 30 minutes.
Detection Chemistry: Uses Endotoxin Green™ with Limulus Amebocyte Lysate.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm with a microplate reader.

E.coli endotoxin dose response was measured in a black/solid bottom 96-well plate using a Gemini microplate reader (Molecular Devices) at Ex/Em=490/525 nm, cutoff=515 nm. As low as 0.001 EU/mL of E.coli Endotoxin can be detected after incubation (n=3).

Catalog	Size	Price	Quantity
60006	100 Tests	Price

Overview

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria. LPS is a potent stimulator of the vertebrate innate immune system and can cause fever, septic shock and eventually death. LPS is also recognized as a biomarker for the detection of bacterial pathogen invasion, and is responsible for the development of inflammatory response and endotoxic shock in extreme cases. Detection of LPS in biological materials, such as protein, peptide or antibody sample, is a critical task in biological manufacturing and processing. Amplite® Fluorimetric Endotoxin Detection Kit uses Endotoxin Green™, a sensitive fluorogenic substrate. Endotoxin Green™ is hydrolyzed in the presence of endotoxins and the Limulus Amebocyte Lysate (LAL), an extract of blood cells from a horseshoe crab. The hydrolyzed product of Endotoxin Green™ generates strong green fluorescence. The endotoxin activity is proportional to the fluorescence intensity resulted from the hydrolysis of Endotoxin Green™. Amplite® Fluorimetric Endotoxin Detection Kit can detect a broad range of endotoxin (from 1 EU/ml to 0.001 EU/ml). It is very sensitive and can detect as low as 0.001 EU/mL of endotoxin within 30 min.

Citations

View all 33 citations: Citation Explorer

A Validation Study of the Limulus Amebocyte Lysate Test as an End-Product Endotoxin Test for Polyvalent Horse Snake Antivenom

Authors:

Sheraba, N. S., Diab, M. R., Yassin, A. S., Amin, M. A., Zedan, H. H.

Journal:

PDA J Pharm Sci Technol (2019): 562-571

Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

Authors:

Uchida, T., Kaku, Y., Hayasaka, H., Kofuji, M., Momose, N., Miyazawa, H., Ueda, Y., Ito, K., Ookawara, S., Morishita, Y.

Journal:

Med Devices (Auckl) (2019): 429-433

Evaluation of a portable test system for assessing endotoxin activity in raw milk

Authors:

Suzuki, Y., Suzuki, K., Shimamori, T., Tsuchiya, M., Niehaus, A., Lakritz, J.

Journal:

J Vet Med Sci (2016): 49-53

Detection of Endotoxin Contamination of Graphene Based Materials Using the TNF-alpha Expression Test and Guidelines for Endotoxin-Free Graphene Oxide Production

Authors:

Mukherjee, S. P., Lozano, N., Kucki, M., Del Rio-Castillo, A. E., Newman, L., Vazquez, E., Kostarelos, K., Wick, P., Fadeel, B.

Journal:

PLoS One (2016): e0166816

COMPARISON OF QuantiFERON(R) TB GOLD TEST RESULTS BEFORE AND AFTER ENDOTOXIN CONTAMINATION

Authors:

Seto, J., Suzuki, Y., Ahiko, T.

Journal:

Kekkaku (2016): 49-52

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black
Instrument specification(s)	Top read mode

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Page updated on October 15, 2026

Component A: Endotoxin Green™	1 Vial
Component B: Endotoxin-Free Water	1 Bottle (25 mL)
Component C: Limulus Amebocyte Lysate	1 Vial
Component D: E.coli Endotoxin Standard	1 Vial (100 EU/mL, 100 µL)
Component E: DMSO	1 Vial (100 µL)