Name: Amplite® Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit
Brand: AAT Bioquest
SKU: 16365
Price: 415 USD
Availability: InStock

Amplite® Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit

Amplite® Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit is a fluorimetric assay kit for quantification of extracellular nitric oxide in media, tissues, and other biological samples.

Detection Principle: Detects extracellular NO using the cell-impermeant DAW-J2 probe.
Signal Advantage: Provides high sensitivity and selectivity for extracellular NO.
Detection Chemistry: DAW-J2 reacts with NO to generate strong red fluorescence.
Instrument Compatibility: Fluorescence detection at Ex/Em 570/610 nm.
Workflow: Compatible with 96- and 384-well microplates.

Nitric oxide dose response was measured with Amplite&trade; Extracelluar Fluorimetric Nitric Oxide Quantitation Kit in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
16365	100 Tests	Price

Overview

Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in biological systems. It has been challenging to detect and understand the role of NO using conventional tools. AAT Bioquest offers a group of NO assay kits for monitoring NO in live cells. This Amplite® Extracellular Fluorimetric Nitric Oxide Quantitation Kit provides a rapid method to monitor NO level in extracellular media, tissues, and other biological solutions and samples. Compared to the commonly used DAF-2 probe, DAW-J2 is used as the cell-impermeant NO sensor in our kit 16365. DAW-J2 detects nitric oxide exclusively in the extracellular environment with high sensitivity and selectivity. The non-fluorescent DAW-J2 probe reacts with NO to generate a strongly red fluorescent product that can be conveniently monitored using a microplate reader.

Citations

View all 2 citations: Citation Explorer

Fluorescent real-time quantitative measurements of intracellular peroxynitrite generation and inhibition

Authors:

Luo, Zhen and Zhao, Qin and Liu, Jixiang and Liao, Jinfang and Peng, Ruogu and Xi, Yunting and Diwu, Zhenjun

Journal:

Analytical biochemistry (2017): 44--48

Inducible Nitric Oxide Synthase (iNOS) Is a Novel Negative Regulator of Hematopoietic Stem/Progenitor Cell Trafficking

Authors:

Adamiak, Mateusz and Abdelbaset-Ismail, Ahmed and Moore, Joseph B and Zhao, J and Abdel-Latif, Ahmed and Wysoczynski, Marcin and Ratajczak, Mariusz Z

Journal:

Stem Cell Reviews and Reports (2016): 1--12

References

View all 139 references: Citation Explorer

Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells

Authors:

Uhlenhut K, Hogger P.

Journal:

Free Radic Biol Med (2012): 2266

Effects of moderate electrical stimulation on reactive species production by primary rat skeletal muscle cells: cross talk between superoxide and nitric oxide production

Authors:

Lambertucci RH, Silveira Ldos R, Hirabara SM, Curi R, Sweeney G, Pithon-Curi TC.

Journal:

J Cell Physiol (2012): 2511

Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate

Authors:

Zhou X, He P.

Journal:

Am J Physiol Heart Circ Physiol (2011): H108

Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERalpha/ERbeta balance in female mice

Authors:

Novensa L, Novella S, Medina P, Segarra G, Castillo N, Heras M, Hermenegildo C, Dantas AP.

Journal:

PLoS One (2011): e25335

Temporal and spatial correlation of platelet-activating factor-induced increases in endothelial [Ca(2)(+)]i, nitric oxide, and gap formation in intact venules

Authors:

Zhou X, He P.

Journal:

Am J Physiol Heart Circ Physiol (2011): H1788

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	570 nm
Emission	610 nm
Cutoff	590 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: DAW-J2	1 Vial
Component B: Assay Buffer	1 Bottle (10 mL)
Component C: DMSO	1 Vial (50 µL)