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Amplite® Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit
Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in biological systems. It has been challenging to detect and understand the role of NO using conventional tools. AAT Bioquest offers a group of NO assay kits for monitoring NO in live cells. This Amplite® Extracellular Fluorimetric Nitric Oxide Quantitation Kit provides a rapid method to monitor NO level in extracellular media, tissues, and other biological solutions and samples. Compared to the commonly used DAF-2 probe, DAW-J2 is used as the cell-impermeant NO sensor in our kit 16365. DAW-J2 detects nitric oxide exclusively in the extracellular environment with high sensitivity and selectivity. The non-fluorescent DAW-J2 probe reacts with NO to generate a strongly red fluorescent product that can be conveniently monitored using a microplate reader.
Nitric oxide dose response was measured with Amplite™ Extracelluar Fluorimetric Nitric Oxide Quantitation Kit in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices). 
Nitric oxide dose response was measured with Amplite™ Extracelluar Fluorimetric Nitric Oxide Quantitation Kit in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices). 
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16365100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation570 nm
Emission610 nm
Cutoff590 nm
Recommended plateSolid black
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Page updated on October 13, 2025