Name: Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 11100
Price: 232 USD
Availability: InStock

Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit

Blue Fluorescence

Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative. The Amplite® fluorescamine protein assay kit provides a simple method for quantifying protein concentration in solutions. This Amplite® fluorescamine protein assay kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. The assay can be completed within 30 minutes. With the Amplite® fluorescamine protein assay kit, as little as 3 ug/mL of BSA can be detected.

BSA dose response was measured on a solid black 96-well plate with Amplite® Fluoremetric Fluorescamine Protein Quantitation Assay Kit.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11100	200 Tests	Price

References

View all 41 references: Citation Explorer

Fluorescence of aromatic amines and their fluorescamine derivatives for detection of explosive vapors

Authors:

Eastwood D, Fern and ez C, Yoon BY, Sheaff CN, Wai CM.

Journal:

Appl Spectrosc (2006): 958

Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent

Authors:

Adamou R, Coly A, Douabale SE, Saleck ML, Gaye-Seye MD, Tine A.

Journal:

J Fluoresc (2005): 679

Determination of 4-amino-m-cresol and 5-amino-o-cresol by high performance liquid chromatography and fluorescence derivatization using fluorescamine

Authors:

Eggenreich K, Zach E, Beck H, Wintersteiger R.

Journal:

J Biochem Biophys Methods (2004): 35

Chiral separation of fluorescamine-labeled amino acids using microfabricated capillary electrophoresis devices for extraterrestrial exploration

Authors:

Skelley AM, Mathies RA.

Journal:

J Chromatogr A (2003): 191

LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations

Authors:

Cesur N, Apak TI, Aboul-Enein HY, Ozkirimli S.

Journal:

J Pharm Biomed Anal (2002): 487

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	380
Emission	470
Cutoff	420
Recommended plate	Solid black

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Page updated on October 13, 2025

Component A: Fluorescamine	1 bottle
Component B: DMSO	1 bottle (5 mL)
Component C: BSA Standard (1 mg/mL)	1 vial (0.5 mL)