Name: Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 11100
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit

Blue Fluorescence

Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence* is a fluorimetric assay kit for quantification of protein concentration in solutions.

Detection Principle: Fluorescamine reacts with primary aliphatic amines to generate fluorescence.
Sensitivity: Detects BSA down to 3 µg/mL.
Detection Chemistry: Uses fluorescamine to label amine-containing peptides and proteins.
Instrument Compatibility: Supports 96- and 384-well plates with fluorescence detection at Ex/Em 380/470 nm.
Workflow: Completes in 30 minutes with no separation steps.

BSA dose response was measured on a solid black 96-well plate with Amplite® Fluoremetric Fluorescamine Protein Quantitation Assay Kit.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11100	200 Tests	Price

Overview

Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative. The Amplite® fluorescamine protein assay kit provides a simple method for quantifying protein concentration in solutions. This Amplite® fluorescamine protein assay kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. The assay can be completed within 30 minutes. With the Amplite® fluorescamine protein assay kit, as little as 3 ug/mL of BSA can be detected.

Citations

View all 2 citations: Citation Explorer

Polydopamine Modified with Brain Targeting Peptide Rabies Virus Glycoprotein for Treatment of Alzheimer’s Disease by Inhibiting Oxidative Stress and Inflammatory Response

Authors:

Chu, Heling and Sun, Yihao and Huang, Chuyi and Wang, Lei and Guo, Qihao and Jiang, Lixian

Journal:

International Journal of Nanomedicine (2026): 1--19

Covalent vaccination with Trypanosoma cruzi Tc24 induces catalytic antibody production

Authors:

Gunter, Sarah M and Versteeg, Leroy and Jones, Kathryn M and Brian, Keegan P and Strych, Ulrich and Bottazzi, Maria Elena and Hotez, Peter J and Brown, Eric L

Journal:

Parasite immunology (2018): e12585

References

View all 41 references: Citation Explorer

Fluorescence of aromatic amines and their fluorescamine derivatives for detection of explosive vapors

Authors:

Eastwood D, Fern and ez C, Yoon BY, Sheaff CN, Wai CM.

Journal:

Appl Spectrosc (2006): 958

Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent

Authors:

Adamou R, Coly A, Douabale SE, Saleck ML, Gaye-Seye MD, Tine A.

Journal:

J Fluoresc (2005): 679

Determination of 4-amino-m-cresol and 5-amino-o-cresol by high performance liquid chromatography and fluorescence derivatization using fluorescamine

Authors:

Eggenreich K, Zach E, Beck H, Wintersteiger R.

Journal:

J Biochem Biophys Methods (2004): 35

Chiral separation of fluorescamine-labeled amino acids using microfabricated capillary electrophoresis devices for extraterrestrial exploration

Authors:

Skelley AM, Mathies RA.

Journal:

J Chromatogr A (2003): 191

LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations

Authors:

Cesur N, Apak TI, Aboul-Enein HY, Ozkirimli S.

Journal:

J Pharm Biomed Anal (2002): 487

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	380
Emission	470
Cutoff	420
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 5, 2026

Component A: Fluorescamine	1 bottle
Component B: DMSO	1 bottle (5 mL)
Component C: BSA Standard (1 mg/mL)	1 vial (0.5 mL)