Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Formaldehyde Quantitation Kit

Green Fluorescence

Amplite® Fluorimetric Formaldehyde Quantitation Kit Green Fluorescence is a fluorimetric assay kit for quantification of formaldehyde in a microplate format.

Detection Principle: Generates fluorescence upon reaction with formaldehyde.
Detection Chemistry: Uses the proprietary AldeLight™ Green fluorogenic dye.
Workflow: Mix-and-read format with no separation step.
Instrument Compatibility: Fluorescence detection at Ex/Em 410/525 nm with a microplate reader.

Formaldehyde dose response was measured in a black/solid
bottom 96-well plate using a Gemini microplate reader (Molecular
Devices) at Ex/Em=410/525 nm, cutoff=495 nm.

Protocol

SDS

Catalog	Size	Price	Quantity
10057	200 Tests	Price

Overview

Formaldehyde is a naturally occurring substance. Natural processes in the upper atmosphere may contribute up to 90 percent of the total formaldehyde in the environment. Formaldehyde, as well as its oligomers and hydrates are rarely encountered in living organisms. Methanogenesis proceeds via the equivalent of formaldehyde, but this one-carbon species is masked as a methylene group in methanopterin. Formaldehyde is the primary cause of methanol's toxicity, since methanol is metabolized into toxic formaldehyde by alcohol dehydrogenase. Our Amplite® Fluorimetric Formaldehyde Quantitation Kit are used for quantifying formaldehyde. The kit uses a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with formaldehyde. This fluorimetric kit provides a sensitive mix-and-read method to detect formaldehyde. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read with a fluorescence microplate reader.

Citations

View all 5 citations: Citation Explorer

High-Throughput Assay of Cytochrome P450-Dependent Drug Demethylation Reactions and Its Use to Re-evaluate the Pathways of Ketamine Metabolism

Authors:

Davydova, Nadezhda Y and Hutner, David A and Gaither, Kari A and Singh, Dilip Kumar and Prasad, Bhagwat and Davydov, Dmitri R

Biological Activity of Peptide-conjugated Polyion Complex Matrices Consisting of Alginate and Chitosan

Authors:

Fujimori, Chikara and Kumai, Jun and Nakamura, Kyotaro and Gu, Yingzi and Katagiri, Fumihiko and Hozumi, Kentaro and Kikkawa, Yamato and Nomizu, Motoyoshi

Journal:

Peptide Science (2016)

Integrated self-assembling drug delivery system possessing dual responsive and active targeting for orthotopic ovarian cancer theranostics

Authors:

Lin, Chun-Jui and Kuan, Chen-Hsiang and Wang, Li-Wen and Wu, Hsi-Chin and Chen, Yunching and Chang, Chien-Wen and Huang, Rih-Yang and Wang, Tzu-Wei

Journal:

Biomaterials (2016): 12--26

Hepatic Deficiency of Augmenter of Liver Regeneration Exacerbates Alcohol-Induced Liver Injury and Promotes Fibrosis in Mice

Authors:

Kumar, Sudhir and Wang, Jiang and Rani, Richa and G, undefined and hi, Ch and rashekhar R, undefined

Journal:

PloS one (2016): e0147864

Fiber-optic protease sensor based on the degradation of thin gelatin films

Authors:

Schyrr, Bastien and Boder-Pasche, Stéphanie and Ischer, Réal and Smajda, Rita and Voirin, Guy

Journal:

Sensing and Bio-Sensing Research (2015): 65--73

References

View all 23 references: Citation Explorer

Enzymatic method for formaldehyde assay based on formaldehyde dehydrogenase from the recombinant yeast Hansenula polymorpha

Authors:

Demkiv OM, Haida HZ, Honchar MV.

Journal:

Ukr Biokhim Zh (2009): 111

Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde

Authors:

Khlupova M, Kuznetsov B, Demkiv O, Gonchar M, Csoregi E, Shleev S.

Journal:

Talanta (2007): 934

The implausibility of systemic genotoxic effects measured by the comet assay in rats exposed to formaldehyde

Authors:

Speit G., undefined

Journal:

J Proteome Res (2006): 2523

Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane

Authors:

Ben Ali M, Korpan Y, Gonchar M, El'skaya A, Maaref MA, Jaffrezic-Renault N, Martelet C.

Journal:

Biosens Bioelectron (2006): 575

A new enzymo-chemical method for simultaneous assay of methanol and formaldehyde

Authors:

Gonchar MV, Grabek D, Oklejewich B, Pavlishko HM, Shamlian OV, Sybirny VA, Kotylak Z, Rudke K, Csoregi E, Sibirny AA.

Journal:

Ukr Biokhim Zh (2005): 146

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	410 nm
Emission	525 nm
Cutoff	495 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: AldeLight™ Green	1 vial
Component B: Assay Buffer	1 bottle (30 mL)
Component C: 37.2% Formaldehyde Standard (12.3 M)	1 vial (100 µL)
Component D: DMSO	1 vial (100 µL)