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Amplite® Fluorimetric Formaldehyde Quantitation Kit
Green Fluorescence
Formaldehyde is a naturally occurring substance. Natural processes in the upper atmosphere may contribute up to 90 percent of the total formaldehyde in the environment. Formaldehyde, as well as its oligomers and hydrates are rarely encountered in living organisms. Methanogenesis proceeds via the equivalent of formaldehyde, but this one-carbon species is masked as a methylene group in methanopterin. Formaldehyde is the primary cause of methanol's toxicity, since methanol is metabolized into toxic formaldehyde by alcohol dehydrogenase. Our Amplite® Fluorimetric Formaldehyde Quantitation Kit are used for quantifying formaldehyde. The kit uses a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with formaldehyde. This fluorimetric kit provides a sensitive mix-and-read method to detect formaldehyde. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read with a fluorescence microplate reader.
Formaldehyde dose response was measured in a black/solid
bottom 96-well plate using a Gemini microplate reader (Molecular
Devices) at Ex/Em=410/525 nm, cutoff=495 nm.
Formaldehyde dose response was measured in a black/solid
bottom 96-well plate using a Gemini microplate reader (Molecular
Devices) at Ex/Em=410/525 nm, cutoff=495 nm.
CatalogSize
Price
Quantity
10057200 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation410 nm
Emission525 nm
Cutoff495 nm
Recommended plateSolid black
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Page updated on October 13, 2025