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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway. It is critical metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH), and for the production of pentose sugars. The production of NADPH is of great importance for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The NADPH also maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Deficiencies in G6PD predispose individuals to non-immune hemolytic anemia. AAT Bioquest's Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit provides a simple, sensitive and rapid fluorescence-based method for detecting G6PD in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, G6PD activity is proportionally related to the concentration of NADPH that is specifically monitored by a fluorogenic NADPH sensor to yield a highly red fluorescence product. The fluorescence signal can be read with a fluorescence microplate reader. With the G6PD assay kit, we were able to detect as little as 1 mU/ml G6PD in a 100 µL reaction volume.
G6PD dose response was measured with Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader. The concentration of the Glucose-6-Phosphate Dehydrogenase in X-axis is based on the 50 uL standard, the final in-well concentration should be 1/2 for each point.
G6PD dose response was measured with Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader. The concentration of the Glucose-6-Phosphate Dehydrogenase in X-axis is based on the 50 uL standard, the final in-well concentration should be 1/2 for each point.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13806200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 4, 2025