Name: Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Brand: AAT Bioquest
SKU: 13806
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit is a fluorimetric assay kit for quantification of G6PD activity in serum, plasma, urine, and cell culture samples.

Detection Principle: Measures NADPH generated by G6PD activity.
Sensitivity: Detects G6PD down to 1 mU/mL.
Detection Chemistry: Uses a fluorogenic NADPH sensor for red fluorescence.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.

G6PD dose response was measured with Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader. The concentration of the Glucose-6-Phosphate Dehydrogenase in X-axis is based on the 50 uL standard, the final in-well concentration should be 1/2 for each point.

Protocol

SDS

Catalog	Size	Price	Quantity
13806	200 Tests	Price

Overview

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway. It is critical metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH), and for the production of pentose sugars. The production of NADPH is of great importance for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The NADPH also maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Deficiencies in G6PD predispose individuals to non-immune hemolytic anemia. AAT Bioquest's Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit provides a simple, sensitive and rapid fluorescence-based method for detecting G6PD in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, G6PD activity is proportionally related to the concentration of NADPH that is specifically monitored by a fluorogenic NADPH sensor to yield a highly red fluorescence product. The fluorescence signal can be read with a fluorescence microplate reader. With the G6PD assay kit, we were able to detect as little as 1 mU/ml G6PD in a 100 µL reaction volume.

References

View all 71 references: Citation Explorer

The microsomal enzyme 17beta-hydroxysteroid dehydrogenase 3 faces the cytoplasm and uses NADPH generated by glucose-6-phosphate dehydrogenase

Authors:

Legeza B, Balazs Z, Nashev LG, Odermatt A.

Journal:

Endocrinology (2013): 205

Molecular characterization of glucose-6-phosphate dehydrogenase deficient variants in Baghdad city - Iraq

Authors:

Al-Musawi BM, Al-Allawi N, Abdul-Majeed BA, Eissa AA, Jubrael JM, Hamamy H.

Journal:

BMC Blood Disord (2012): 4

High prevalence of hemoglobin disorders and glucose-6-phosphate dehydrogenase (G6PD) deficiency in the Republic of Guinea (West Africa)

Authors:

Millimono TS, Loua KM, Rath SL, Relvas L, Bento C, Diakite M, Jarvis M, Daries N, Ribeiro LM, Manco L, Kaeda JS.

Journal:

Hemoglobin (2012): 25

Effects of laparoscopic Roux-en-Y gastric bypass on glucose-6 phosphate dehydrogenase activity in obese type 2 diabetics

Authors:

Schneider AM, Rawat D, Weinstein LS, Gupte SA, Richards WO.

Journal:

Surg Endosc (2012): 823

A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency

Authors:

Shah SS, Diakite SA, Traore K, Diakite M, Kwiatkowski DP, Rockett KA, Wellems TE, Fairhurst RM.

Journal:

Sci Rep (2012): 299

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Enzyme Probe	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NADP	1 vial
Component D: G6PD Standard	10 U/vial