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Amplite® Fluorimetric Glucose and Sucrose Assay Kit
Glucose, a key molecule in cellular energy metabolism, primarily functions during cellular respiration. It undergoes glycolytic breakdown for immediate ATP production or is converted into glycogen for energy storage. Sucrose, a disaccharide, is broken down into glucose and fructose by the enzyme invertase. The Amplite® Fluorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium. This kit is useful for assessing aerobic glycolysis, pyruvate kinase activity, and metabolic profiles in different biological matrices. It is also employed for determining protein and sugar concentrations in food samples, as well as monitoring plasma insulin and glucose levels in murine models. To measure glucose levels, glucose oxidase specifically oxidizes free glucose, producing a fluorimetric product (Ex/Em = 571/584 nm) that is proportional to the glucose present. To measure sucrose levels, invertase is added to the reaction to convert sucrose into free glucose and fructose, allowing for the measurement of the total glucose level. The sucrose level is then calculated by subtracting the free glucose from the total glucose.
Sucrose dose response was measured with Amplite® Colorimetric Glucose and Sucrose Assay Kit on a 96-well clear bottom microplate after 30 minutes of incubation using a Gemini microplate reader (Molecular Devices) at Ex/Em = 540/590 nm, cutoff = 570.
Sucrose dose response was measured with Amplite® Colorimetric Glucose and Sucrose Assay Kit on a 96-well clear bottom microplate after 30 minutes of incubation using a Gemini microplate reader (Molecular Devices) at Ex/Em = 540/590 nm, cutoff = 570.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
40010100 Tests
Price
 
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540
Emission590
Cutoff570
Recommended plateSolid black
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Page updated on May 4, 2026