Name: Amplite® Fluorimetric Glucose and Sucrose Assay Kit
Brand: AAT Bioquest
SKU: 40010
Price: 516 USD
Availability: InStock

Amplite® Fluorimetric Glucose and Sucrose Assay Kit

Amplite® Fluorimetric Glucose and Sucrose Assay Kit is a fluorimetric assay kit for quantification of glucose and sucrose in serum, plasma, body fluids, food, tissue extracts, and growth medium.

Detection Principle: Measures free glucose through glucose oxidase-mediated fluorescence.
Specificity: Determines sucrose after invertase conversion to glucose and fructose.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with microplate reader.
Plate Format: Recommended for solid black microplates.

Sucrose dose response was measured with Amplite® Colorimetric Glucose and Sucrose Assay Kit on a 96-well clear bottom microplate after 30 minutes of incubation using a Gemini microplate reader (Molecular Devices) at Ex/Em = 540/590 nm, cutoff = 570.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
40010	100 Tests	Price

Overview

Glucose, a key molecule in cellular energy metabolism, primarily functions during cellular respiration. It undergoes glycolytic breakdown for immediate ATP production or is converted into glycogen for energy storage. Sucrose, a disaccharide, is broken down into glucose and fructose by the enzyme invertase. The Amplite® Fluorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium. This kit is useful for assessing aerobic glycolysis, pyruvate kinase activity, and metabolic profiles in different biological matrices. It is also employed for determining protein and sugar concentrations in food samples, as well as monitoring plasma insulin and glucose levels in murine models. To measure glucose levels, glucose oxidase specifically oxidizes free glucose, producing a fluorimetric product (Ex/Em = 571/584 nm) that is proportional to the glucose present. To measure sucrose levels, invertase is added to the reaction to convert sucrose into free glucose and fructose, allowing for the measurement of the total glucose level. The sucrose level is then calculated by subtracting the free glucose from the total glucose.

References

View all 50 references: Citation Explorer

Investigating the production and synergistic antibacterial activity of bacteriocin-like substance from Brevibacillus laterosporus SA-14 (TISTR 2453) for enhanced wound healing.

Authors:

Madoromae, Husna and Atipairin, Apichart and Tayeh, Malatee and Lertcanawanichakul, Monthon

Journal:

Heliyon (2025): e42510

Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3.

Authors:

Raethong, Nachon and Chamtim, Pitakthai and Thananusak, Roypim and Whanmek, Kanyawee and Santivarangkna, Chalat

Journal:

Heliyon (2025): e41879

Sampling-free investigation of microbial carbon source preferences on renewable feedstocks via online monitoring of oxygen transfer rate.

Authors:

Grebe, Luca Antonia and Richter, Paul and Altenkirch, Torben and Mann, Marcel and Müller, Markus Jan and Büchs, Jochen and Magnus, Jørgen Barsett

Journal:

Bioprocess and biosystems engineering (2025): 413-425

GRAIN SIZE ON CHROMOSOME 2 orchestrates phytohormone, sugar signaling and energy metabolism to confer thermal resistance in rice.

Authors:

Li, Guangyan and Wang, Huanran and Li, Hubo and Feng, Baohua and Fu, Weimeng and Ma, Jiaying and Li, Juncai and Wu, Zhihai and Islam, Md Rezaul and Chen, Tingting and Zhang, Hongcheng and Wei, Haiyan and Tao, Longxing and Fu, Guanfu

Journal:

Physiologia plantarum (2025): e70113

Transcriptomic analysis reveals long non-coding RNA involved in the key metabolic pathway in response to Botrytis cinerea in kiwifruit.

Authors:

Ma, Yijia and Zeng, Tianjing and Li, Zhexin and Jue, Dengwei and Sui, Yuan and Wang, Xu and Zhong, Hongpan and Yang, Jiaqi

Journal:

BMC plant biology (2025): 474

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540
Emission	590
Cutoff	570
Recommended plate	Solid black

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Page updated on October 24, 2026

Component A: Amplite® Red	1 Vial
Component B: Sucrose Assay Buffer	25 mL
Component C: Invertase	1 Vial
Component D: Glucose Enzyme Mix	1 Bottle
Component E: Sucrose Standard	1 Vial
Component F: DMSO	1 Vial