Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glucose Oxidase Assay Kit

Red Fluorescence

The glucose oxidase is a dimeric protein that catalyzes the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. It is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, glucose oxidase is commonly used in biosensors to detect glucose. The Amplite® Glucose Oxidase Assay Kit provides a quick and sensitive method for the measurement of glucose oxidase in solution. It can be performed in a convenient 96-well or 384-well microtiter-plate format and is easily adapted to automation without a separation step. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Fluorimetric Glucose Oxidase Assay Kit, we have detected as little as 0.05 mU/mL glucose oxidase in a 100 µL reaction volume.

Glucose oxidase dose response was measured with Amplite® Fluorimetric Glucose Oxidase Assay Kit in a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11300	500 Tests	Price

Citations

View all 3 citations: Citation Explorer

A reassessment of the carnivorous status of salmonids: hepatic glucokinase is expressed in wild fish in Kerguelen Islands

Authors:

Marandel, Lucie and Gaudin, Philippe and Gu{\'e}raud, Fran{\c{c}}ois and Glise, St{\'e}phane and Herman, Alexandre and Plagnes-Juan, Elisabeth and V{\'e}ron, Vincent and Panserat, St{\'e}phane and Labonne, Jacques

Journal:

Science of the Total Environment (2018): 276--285

A reassessment of the carnivorous status of salmonids: Hepatic glucokinase is expressed in wild fish in Kerguelen Islands

Authors:

Mar, undefined and el, Lucie and Gaudin, Philippe and Guéraud, Fran&ccaron;ois and Glise, Stéphane and Herman, Alex and re , undefined and Plagnes-Juan, Elisabeth and Véron, Vincent and Panserat, Stéphane and Labonne, Jacques

Journal:

Science of The Total Environment (2018): 276--285

Overcoming 5-Fu resistance in human non-small cell lung cancer cells by the combination of 5-Fu and cisplatin through the inhibition of glucose metabolism

Authors:

Zhao, Jun-gang and Ren, Kai-ming and Tang, Jun

Journal:

Tumor Biology (2014): 12305--12315

References

View all 29 references: Citation Explorer

Factors affecting accuracy and time requirements of a glucose oxidase-peroxidase assay for determination of glucose

Authors:

Hall MB, Keuler NS.

Journal:

J AOAC Int (2009): 50

Conformation and activity dependent interaction of glucose oxidase with CdTe quantum dots: towards developing a nanoparticle based enzymatic assay

Authors:

Priyam A, Chatterjee A, Bhattacharya SC, Saha A.

Journal:

Photochem Photobiol Sci (2009): 362

Using an indicator displacement assay to monitor glucose oxidase activity in blood serum

Authors:

Zhang T, Anslyn EV.

Journal:

Org Lett (2007): 1627

Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening

Authors:

Funabashi H, Tanaka Y, Imamura Y, Mie M, Manabe T, Tanaka H, Takahashi T, H and a H, Aizawa M, Kobatake E.

Journal:

Biosens Bioelectron (2006): 1675

1,4-Benzoquinone-based electrophoretic assay for glucose oxidase

Authors:

Urban PL, Goodall DM, Bergstrom ET, Bruce NC.

Journal:

Anal Biochem (2006): 35

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 24, 2025

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Horseradish Peroxidase (HRP)	1 vial
Component D: Glucose Oxidase	1 vial (100 units)
Component E: DMSO	1 vial (200 µL)
Component F: Glucose	1 vial