Amplite® Fluorimetric Glucose Quantitation Kit

Amplite® Fluorimetric Glucose Quantitation Kit is a fluorimetric assay kit for quantification of glucose in serum, plasma, body fluids, food, and growth media.

Detection Principle: Measures hydrogen peroxide generated by glucose oxidase.
Detection Chemistry: Uses Amplite® Red with horseradish peroxidase coupling.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.

Glucose dose response was measured with Amplite® Fluorimetric Glucose Quantitation Kit on a 96-well black plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
40005	500 Tests	Price

Overview

Glucose, a monosaccharide, is the most important carbohydrate in biology. It is a source of energy and metabolic intermediate for cell growth. Glucose is one of the main products of photosynthesis and starts cellular respiration in both prokaryotes and eukaryotes. Glucose level is a key diagnostic parameter for many metabolic disorders. This glucose assay kit provides a quick and sensitive method for the measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). The kit uses our Amplite®Red substrate that making the kit recordable in a dual more, either fluorimetric or colorimetric readout. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays in a wide variety of applications that require the measurement of glucose. For example, the assay might be suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. It might also be used for monitoring glucose transporters.

Citations

View all 20 citations: Citation Explorer

ALKBH3-regulated m1A of ALDOA potentiates glycolysis and doxorubicin resistance of triple negative breast cancer cells

Authors:

Deng, Yuhua and Chen, Zhiyan and Chen, Peixian and Xiong, Yaming and Zhang, Chuling and Wu, Qiuyuan and Huang, Huiqi and Yang, Shuqing and Zhang, Kun and He, Tiancheng and others,

Journal:

Acta Pharmaceutica Sinica B (2025)

Molecular programming of the hepatic lipid metabolism via a parental high carbohydrate and low protein diet in rainbow trout

Authors:

Callet, Th{\'e}r{\`e}se and Li, Hongyan and Heraud, C{\'e}cile and Larroquet, Laurence and Lanuque, Anthony and Sandres, Franck and Terrier, Fr{\'e}d{\'e}ric and Surget, Anne and Corraze, Genevi{\`e}ve and Panserat, St{\'e}phane and others,

Journal:

animal (2022): 100670

RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53

Authors:

Jacquier, Valentin and Gitenay, Delphine and Fritsch, Samuel and Bonnet, Sandrine and Gy{\H{o}}rffy, Bal{\'a}zs and Jalaguier, St{\'e}phan and Linares, Laetitia K and Cavaill{\`e}s, Vincent and Teyssier, Catherine

Journal:

Cellular and Molecular Life Sciences (2022): 1--17

No adverse effect of a maternal high carbohydrate diet on their offspring, in rainbow trout (Oncorhynchus mykiss)

Authors:

Callet, Therese and Li, Hongyan and Surget, Anne and Terrier, Frederic and Sandres, Franck and Lanuque, Anthony and Panserat, Stephane and Marandel, Lucie

Journal:

PeerJ (2021): e12102

Exploring the impact of a low-protein high-carbohydrate diet in mature broodstock of a glucose-intolerant teleost, the rainbow trout

Authors:

Callet, Th{\'e}r{\`e}se and Hu, Huihua and Larroquet, Laurence and Surget, Anne and Liu, Jingwei and Plagnes-Juan, Elisabeth and Maunas, Patrick and Turonnet, Nicolas and Mennigen, Jan Alexander and Bobe, Julien and others,

Journal:

Frontiers in physiology (2020): 303

References

View all 21 references: Citation Explorer

Insulin and glucose mediate opposite intracellular ionized magnesium variations in human lymphocytes

Authors:

Delva P, Degan M, Trettene M, Lechi A.

Journal:

J Endocrinol (2006): 711

Recombinant human glucose-6-phosphate dehydrogenase. Evidence for a rapid-equilibrium random-order mechanism

Authors:

Wang XT, Au SW, Lam VM, Engel PC.

Journal:

Eur J Biochem (2002): 3417

Fluorescent microplate cell assay to measure uptake and metabolism of glucose in normal human lung fibroblasts

Authors:

Leira F, Louzao MC, Vieites JM, Botana LM, Vieytes MR.

Journal:

Toxicol In Vitro (2002): 267

Glucose-induced alterations of intracellular ionized magnesium in human lymphocytes

Authors:

Delva P, Degan M, Pastori C, Faccini G, Lechi A.

Journal:

Life Sci (2002): 2119

Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test

Authors:

Goi G, Bairati C, Burlina A, Massaccesi L, Monciotti C, Segalini G, Testa R, Lombardo A.

Journal:

Metabolism (2000): 1352

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Horseradish Peroxidase (HRP)	1 vial (10 units)
Component D: Glucose Oxidase	1 vial (100 units)
Component E: DMSO	1 vial (200 µL)
Component F: Glucose	1 vial (144 mg)