Name: Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 10054
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Glutamic Acid Assay Kit

Red Fluorescence

Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of glutamic acid in various biological samples.

Detection Principle: Measures NADPH generated from L-glutamic acid.
Sensitivity: Detects glutamic acid down to 10 µM in a 100 µL reaction.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.
Workflow: Compatible with fluorescence or absorbance microplate readers.

Glutamic acid dose response was measured with Amplite® Glutamic Acid Assay Kit in a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
10054	200 Tests	Price

Overview

Glutamic acid is one of the 20 proteinogenic amino acids. The carboxylate anions and salts of glutamic acid are known as glutamates. Glutamate is an important neurotransmitter which plays a key role in long-term potentiation and is important for learning and memory. Glutamic acid is the precursor of GABA but has somewhat the opposite function. It might play a role in the normal function of the heart and the prostate. As one of the few nutrients that crosses the blood-brain barrier, glutamic acid is used in the treatment of diseases such as depression, ADD and ADHD, fatigue, alcoholism, epilepsy, muscular dystrophy, mental retardation, and schizophrenia. The Amplite® Fluorimetric Glutamic Acid Assay Kit provides a quick and sensitive method for the measurement of glutamic acid in various biological samples. In the assay, the coupled enzyme system catalyzes the reaction between L-glutamic acid and NADP to produce NADPH, which is specifically recognized by our NADPH sensor and recycled back to NADP. A red flurescence product is produced during the reaction. The signal can be read by either a fluorescence microplate reader or an absorbance microplate reader. With our Amplite® Fluorimetric Glutamic Acid Kit, we have detected as little as 10 µM glutamic acid in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications that require the measurement of glutamic acid.

Citations

View all 2 citations: Citation Explorer

Experimentally evolved Staphylococcus aureus shows increased survival in the presence of Pseudomonas aeruginosa by acquiring mutations in the amino acid transporter, GltT

Authors:

Alexander, Ashley M and Luu, Justin M and Raghuram, Vishnu and Bottacin, Giulia and van Vliet, Simon and Read, Timothy D and Goldberg, Joanna B

Journal:

Microbiology (2024): 001445

Presenilin-1/γ-secretase controls glutamate release, tyrosine phosphorylation, and surface expression of N-methyl-d-aspartate receptor (NMDAR) subunit GluN2B

Authors:

Xuan, Zhao and Barthet, Gael and Shioi, Junichi and Xu, Jindong and Georgakopoulos, Anastasios and Bruban, Julien and Robakis, Nikolaos K

Journal:

Journal of Biological Chemistry (2013): 30495--30501

References

View all 74 references: Citation Explorer

The glutamic acid decarboxylase 65 immunoglobulin G subclass profile differs between adult-onset type 1 diabetes and latent autoimmune diabetes in adults (LADA) up to 3 years after clinical onset

Authors:

Hillman M, Torn C, L and in-Olsson M., undefined

Journal:

Clin Exp Immunol (2009): 255

Low agreement between radio binding assays in analyzing glutamic acid decarboxylase (GAD65Ab) autoantibodies in patients classified with type 2 diabetes

Authors:

Daka B, Svensson MK, Lernmark K, Mincheva-Nilsson L, Hallmans G, Rol and sson O., undefined

Journal:

Autoimmunity (2009): 507

Rapid detection of the poly-gamma-D-glutamic acid capsular antigen of Bacillus anthracis by latex agglutination

Authors:

AuCoin DP, Sutherl and MD, Percival AL, Lyons CR, Lovchik JA, Kozel TR.

Journal:

Diagn Microbiol Infect Dis (2009): 229

Acute limbic encephalitis and glutamic acid decarboxylase antibodies: a reality

Authors:

Blanc F, Ruppert E, Kleitz C, Valenti MP, Cretin B, Humbel RL, Honnorat J, Namer IJ, Hirsch E, Manning L, de Seze J.

Journal:

J Neurol Sci (2009): 69

Dynamic regulation of glutamic acid decarboxylase 65 gene expression in rat testis

Authors:

Liu H, Li S, Zhang Y, Yan Y, Li Y.

Journal:

Acta Biochim Biophys Sin (Shanghai) (2009): 545

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Enzyme Mix	1 bottle (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NADP	1 vial
Component D: Glutamic Acid	1 vial
Component E: Dilution Buffer	1 bottle (10 mL)