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Amplite® Fluorimetric Glutamic Acid Assay Kit
Red Fluorescence
Glutamic acid is one of the 20 proteinogenic amino acids. The carboxylate anions and salts of glutamic acid are known as glutamates. Glutamate is an important neurotransmitter which plays a key role in long-term potentiation and is important for learning and memory. Glutamic acid is the precursor of GABA but has somewhat the opposite function. It might play a role in the normal function of the heart and the prostate. As one of the few nutrients that crosses the blood-brain barrier, glutamic acid is used in the treatment of diseases such as depression, ADD and ADHD, fatigue, alcoholism, epilepsy, muscular dystrophy, mental retardation, and schizophrenia. The Amplite® Fluorimetric Glutamic Acid Assay Kit provides a quick and sensitive method for the measurement of glutamic acid in various biological samples. In the assay, the coupled enzyme system catalyzes the reaction between L-glutamic acid and NADP to produce NADPH, which is specifically recognized by our NADPH sensor and recycled back to NADP. A red flurescence product is produced during the reaction. The signal can be read by either a fluorescence microplate reader or an absorbance microplate reader. With our Amplite® Fluorimetric Glutamic Acid Kit, we have detected as little as 10 µM glutamic acid in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications that require the measurement of glutamic acid.
Glutamic acid dose response was measured with Amplite® Glutamic Acid Assay Kit in a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).
Glutamic acid dose response was measured with Amplite® Glutamic Acid Assay Kit in a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
10054200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 12, 2025