Name: Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 10055
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Glutathione Assay Kit

Green Fluorescence

Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence* is a fluorimetric assay kit for quantification of reduced glutathione (GSH) in biological samples.

Detection Principle: Measures NADPH generated from L-glutamic acid.
Sensitivity: Detects glutamic acid down to 10 µM in a 100 µL reaction.
Detection Chemistry: Uses an NADPH sensor with enzymatic recycling.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.
Workflow: Compatible with fluorescence or absorbance microplate readers.

GSH dose responses were measured in a solid black 96-well plate with Amplite® Fluorimetric Glutathione Assay Kit using a NOVOstar microplate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
10055	200 Tests	Price

Overview

The monitoring of reduced and oxidized glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Cysteine metabolism disorders include cystinosis, an autosomal recessive disease produced by a defect in lysosomal transport, and cystinuria, a common heritable disorder of amino acid transport. Cysteine is unique among the amino acids found in proteins. There are a few reagents or assay kits available for quantitating thiols in biological systems. However, all the commercial kits either lack sensitivity or have tedious protocols. Our Amplite® Fluorimetric Glutathione Qutitation Kit provides an ultrasensitive fluorimetric assay to quantitate GSH in sample. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with thiol. The kit provides a sensitive, one-step fluorimetric method to detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read using a fluorescence microplate reader.

Citations

View all 2 citations: Citation Explorer

Xylitol acts as an anticancer monosaccharide to induce selective cancer death via regulation of the glutathione level

Authors:

Tomonobu, Nahoko and Komalasari, Ni Luh Gede Yoni and Sumardika, I Wayan and Jiang, Fan and Chen, Youyi and Yamamoto, Ken-ichi and Kinoshita, Rie and Murata, Hitoshi and Inoue, Yusuke and Sakaguchi, Masakiyo

Journal:

Chemico-Biological Interactions (2020): 109085

Comparison of different methods to study effects of silver nanoparticles on the pro-and antioxidant status of human keratinocytes and fibroblasts

Authors:

Ahlberg, Sebastian and Rancan, Fiorenza and Epple, Matthias and Loza, Kateryna and Höppe, David and Lademann, Jürgen and Vogt, Annika and Kleuser, Burkhard and Gerecke, Christian and Meinke, Martina C

Journal:

Methods (2016)

References

View all 131 references: Citation Explorer

Effects of methodological variation on assessment of riboflavin status using the erythrocyte glutathione reductase activation coefficient assay

Authors:

Hill MH, Bradley A, Mushtaq S, Williams EA, Powers HJ.

Journal:

Br J Nutr (2009): 273

A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase

Authors:

Yi L, Li H, Sun L, Liu L, Zhang C, Xi Z.

Journal:

Angew Chem Int Ed Engl (2009): 4034

A 1,536-well-based kinetic HTS assay for inhibitors of Schistosoma mansoni thioredoxin glutathione reductase

Authors:

Lea WA, Jadhav A, Rai G, Sayed AA, Cass CL, Inglese J, Williams DL, Austin CP, Simeonov A.

Journal:

Assay Drug Dev Technol (2008): 551

A high-throughput reporter gene assay to prove the ability of natural compounds to modulate glutathione peroxidase, superoxide dismutase and catalase gene promoters in V79 cells

Authors:

Ullmann K, Wiencierz AM, Muller C, Thierbach R, Steege A, Toyokuni S, Steinberg P.

Journal:

Free Radic Res (2008): 746

Assay of glutathione in must and wines using capillary electrophoresis and laser-induced fluorescence detection. Changes in concentration in dry white wines during alcoholic fermentation and aging

Authors:

Lavigne V, Pons A, Dubourdieu D.

Journal:

J Chromatogr A (2007): 130

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Thiolite™ Green	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: GSH Standard	1 vial (62 µg)
Component D: DMSO	1 vial (400 µL)