Name: Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 10056
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit

Green Fluorescence

Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* is a fluorimetric assay kit for quantification of GSH, GSSG, and the GSH/GSSG ratio in biological samples.

Detection Principle: Uses thiol-reactive fluorescence to measure GSH and total GSH.
Sensitivity: Detects as little as 1 pmol of cysteine or GSH in a 100 µL assay. AAT Bioquest
Detection Chemistry: Uses Thiolite™ Green with a GSSG-specific probe. AAT Bioquest
Workflow: One-step assay with no separation step.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/520 nm.

GSH and GSSG dose responses were measured with Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit (Cat#10056). Blue: GSH dose responses (0.156 to 10 µM); Red: GSSG dose responses (0.078 to 5 µM GSSG which is equivalent to 0.156 to 10 µM Total GSH).

Protocol

SDS

Catalog	Size	Price	Quantity
10056	200 Tests	Price

Overview

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease. The glutathione redutase recycles GSSG to GSH with simultaneous oxidation of b-nicotinamide adenine dinuclecotide phosphate. The monitoring of GSH/GSSG ratio and the quantification of GSSG in biological samples are essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. There are a few reagents or assay kits available for the quantitation of thiols in biological systems. However, all the commercial kits either lack sensitivity or have tedious protocols. Our Amplite® Fluorimetric GSH/GSSG Ratio Kit provides an ultrasensitive assay to quantitate GSH in the sample. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with thiol. The kit provides a sensitive, one-step fluorimetric method to detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader.

Citations

View all 18 citations: Citation Explorer

Mechanism of matrine inhibiting MRE11 lactylation and DNA damage repair in regulating cholangiocarcinoma sensitivity to I125 radiotherapy

Authors:

Xu, Linfang and Li, Jialing and Li, Xing and Gong, Min and Wang, Guiliang

Journal:

Journal of Radiation Research and Applied Sciences (2026): 102336

A Prussian Blue Nanozyme-Adjuvanted Vaccine Presenting Phosphocholine Antigens for Induction of Immunotolerance in Inflammatory Bowel Disease

Authors:

Sheng, Jingyi and Huang, Yubo and Li, Xinyue and Liu, Yin and Wu, Yuehuang and Zhang, Yuxin and Gu, Ning

Journal:

Advanced Science (2026): e22027

A cotton APS REDUCTASE represses root hair elongation via sulfur assimilation and hydrogen peroxide-mediated cell wall damage

Authors:

Mao, Xiaonan and Wei, Zhenzhen and Xu, Zhenzhen and Qanmber, Ghulam and Liu, Ji and Li, Yonghui and Lu, Lili and Wang, Ye and Peng, Jun and Wang, Zhi

Journal:

Physiologia Plantarum (2024): e14200

YAP promotes the healing of ischemic wounds by reducing ferroptosis in skin fibroblasts through inhibition of ferritinophagy

Authors:

Cao, Guoqi and Yin, Siyuan and Ma, Jiaxu and Lu, Yongpan and Song, Ru and Wu, Zhenjie and Liu, Chunyan and Liu, Jian and Wu, Peng and Sun, Rui and others,

Journal:

Heliyon (2024)

Glutathione S-transferase theta 1 protects against colitis through goblet cell differentiation via interleukin-22

Authors:

Kim, Jae Hyeon and Ahn, Jae Bum and Kim, Da Hye and Kim, Soochan and Ma, Hyun Woo and Che, Xiumei and Seo, Dong Hyuk and Kim, Tae Il and Kim, Won Ho and Cheon, Jae Hee and others,

Journal:

The FASEB Journal (2020)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	520 nm
Cutoff	510 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Thiolite™ Green	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: GSH Standard	1 vial (62 μg)
Component D: DMSO	1 vial (400 μL)
Component E: GSSG Probe	1 bottle (lyophilized powder)
Component F: GSSG Standard	1 vial (124 μg)