Name: Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 11560
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit

Blue Fluorescence

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence* is a fluorimetric assay kit for quantification of glutathione peroxidase activity in biological samples.

Detection Principle: Measures NADP⁺ generated during GPx-catalyzed glutathione oxidation.
Sensitivity: Detects GPx activity down to 1.25 mU/mL.
Detection Chemistry: Uses Quest Fluor™ NADP Probe for NADP⁺-specific fluorescence.
Instrument Compatibility: Fluorescence detection at Ex/Em 420/480 nm.
Plate Format: Compatible with 96- and 384-well microplates.

Glutathione Peroxidase (GPx) dose response was measured with Amplite® Fluorimetric Glutathione Peroxidase Assay Kit (Cat#11560) on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11560	200 Tests	Price

Overview

Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage. Glutathione peroxidase plays an important role in reducing organic hydroperoxides such as lipid hydroperoxides to their corresponding alcohols, or reducing free hydrogen peroxide to water. Glutathione peroxidase guards against oxidative damage to cell membranes and other oxidant-sensitive sites in cells. The altered glutathione peroxidase levels correlate with lesions caused by many common and complex diseases. Glutathione peroxidase level is measured in biological samples as a potential indicator for the potential treatment of cancer, diabetes, neurodegenerative and cardiovascular diseases. AAT Bioquest's Fluorimetric Glutathione Peroxidase Assay Kit offers a sensitive fluorimetric assay for measuring glutathione peroxidase levels in biological samples. This assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG) catalyzed by glutathione peroxidase. The generated GSSG is recycled to its reduced state GSH by glutathione reductase (GR) and NADPH to generate NADP⁺ that can be specifically monitored using Quest Fluor™ NADP Probe, our newly developed proprietary NADP sensor. The NADP sensor reacts only with NADP to generate a fluorescent product. The fluorescence signal can be measured with a fluorescence microplate reader and is directly proportional to the glutathione peroxidase activity. Compared to other commercial kits that measure the decrease in absorbance of NADPH at 340 nm, our Quest Fluor™ NADP Probe can be used for quantify NADP level directly. With this fluorimetric glutathione peroxidase assay, as low as 1.25 mU/mL glutathione peroxidase can be detected in a 155 µL reaction volume.

Citations

View all 4 citations: Citation Explorer

Chronic Low-Dose Gold Nanoparticle Exposure Induces Persistent Alterations in Mitochondrial Respiration in a Physicochemical-Property Dependent Manner

Authors:

Nunes, Abner and Falagan-Lotsch, Priscila

Journal:

ACS Applied Materials \& Interfaces (2026)

Erinacine A-Enriched Hericium erinaceus Mycelium Ethanol Extract Lessens Cellular Damage in Cell and Drosophila Models of Spinocerebellar Ataxia Type 3 by Improvement of Nrf2 Activation

Authors:

Wu, Yu-Ling and Sun, Hai-Lun and Chang, Jui-Chih and Lin, Wei-Yong and Chen, Pei-Yin and Chen, Chin-Chu and Lee, Li-Ya and Li, Chien-Chun and Hsieh, Mingli and Chen, Haw-Wen and others,

Journal:

Antioxidants (2024): 1495

Cloak Scavenges the Reactive Oxygen Species around the Larvae of Drino inconspicuoides (Diptera: Tachinidae)

Authors:

Zhang, Kai and Nakamura, Satoshi and Furukawa, Seiichi

Journal:

Insects (2023): 602

Histidine-rich glycoprotein possesses antioxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton's reaction

Authors:

Wake, Hidenori and Takahashi, Yohei and Yoshii, Yukinori and Gao, Shangze and Mori, Shuji and Wang, Dengli and Teshigawara, Kiyoshi and Nishibori, Masahiro

Journal:

Free Radical Research (2020): 649--661

References

View all 52 references: Citation Explorer

Construction of a highly stable artificial glutathione peroxidase on a protein nanoring

Authors:

Miao L, Zhang X, Si C, Gao Y, Zhao L, Hou C, Shoseyov O, Luo Q, Liu J.

Journal:

Org Biomol Chem (2014): 362

Glutathione peroxidase-1 is required for self-renewal of murine embryonic stem cells

Authors:

Wang QY, Liu ZS, Wang J, Wang HX, Li A, Yang Y, Wang XZ, Zhao YQ, Han QY, Cai H, Liang B, Song N, Li WH, Li T.

Journal:

Biochem Biophys Res Commun (2014): 454

Protective effect of ((4-tert-butylcyclohexylidene) methyl) (4-methoxystyryl) sulfide, a novel unsymmetrical divinyl sulfide, on an oxidative stress model induced by sodium nitroprusside in mouse brain: involvement of glutathione peroxidase activity

Authors:

Ianiski FR, Alves CB, Bassaco MM, Silveira CC, Luchese C.

Journal:

J Pharm Pharmacol. (2014)

Expression of glutathione peroxidase 1 in the spheno-occipital synchondrosis and its role in ROS-induced apoptosis

Authors:

Koretsi V, Kirschneck C, Proff P, Romer P.

Journal:

Eur J Orthod. (2014)

Deletion of the four phospholipid hydroperoxide glutathione peroxidase genes accelerates aging in Caenorhabditis elegans

Authors:

Sakamoto T, Maebayashi K, Nakagawa Y, Imai H.

Journal:

Genes Cells (2014): 778

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	420 nm
Emission	480 nm
Cutoff	430 nm
Recommended plate	Solid black

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Email	sales@aatbio.com
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Page updated on October 8, 2026

Component A: Glutathione Peroxidase Standard	1 vial (0.5 U/vial)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: Enzyme Mix	2 bottles (lyophilized powder)
Component D: GSH	1 vial (3 mg/vial)
Component E: GPx Substrate	1vial (11 µL/vial)
Component F: Quest Fluor™ NADP Probe	1 bottle (5 mL)
Component G: NADP Assay Solution	1 bottle (5 mL)
Component H: Enhancer Solution	1 bottle (3.5 mL)