Name: Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Brand: AAT Bioquest
SKU: 13837
Price: 415 USD
Availability: InStock

Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit is a fluorimetric assay kit for quantification of G3P in a microplate format.

Detection Principle: Measures H₂O₂ generated by G3P oxidase-mediated enzyme coupling.
Sensitivity: Detects G3P down to 0.41 µM (41 pmol) in a 100 µL assay.
Detection Chemistry: Uses Amplite® Red substrate for fluorescent detection.
Workflow: Mix-and-read format compatible with HTS applications.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.

Glycerol-3-phosphate dose response was obtained with Amplite® Fluorimetric Glycerol 3-Phosphate Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13837	200 Tests	Price

Overview

Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway. Animals, fungi, and plants use G3P to produce ATP. It is used to regenerate NAD+ in brain and skeletal muscle cells. G3P has been linked to lipid imbalance diseases such as obesity. Amplite® Glycerol 3-Phosphate Assay Kit provides one of the most sensitive methods for quantifying G3P. The kit uses Amplite® Red substrate to quantify G3P concentration, which is proportional to the production of hydrogen peroxide in the G3P oxidase-mediated enzyme coupling reactions. The kit is an optimized "mix and read" format that is compatible with HTS applications. It detects as little as 41 picomole G3P in 100 µL solution (0.41 µM) as shown in Figure 1. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format without a separation step. Its signal can be easily read with a fluorescence microplate reader.

Citations

View all 6 citations: Citation Explorer

A redox-sensitive phosphatase regulates glycolysis as a metabolic switch in the bacterial inner membrane

Authors:

Zheng, Lei and Niu, Wei and Xie, Xianfa and Vu, Trung and Du, Guangwei

Journal:

Science Advances (2026): eaea8724

Glucose enhances catecholamine-stimulated lipolysis via increased glycerol-3-phosphate synthesis in 3T3-L1 adipocytes and rat adipose tissue

Authors:

Takeuchi, Nodoka and Higashida, Kazuhiko and Li, Xi and Nakai, Naoya

Journal:

Molecular Biology Reports (2021): 6269--6276

Glycerol suppresses glucose consumption in trypanosomes through metabolic contest

Authors:

Allmann, Stefan and Wargnies, Marion and Plazolles, Nicolas and Cahoreau, Edern and Biran, Marc and Morand, Pauline and Pineda, Erika and Kulyk, Hanna and Asencio, Corinne and Villafraz, Oriana and others,

Journal:

PLoS Biology (2021): e3001359

“Metabolic contest”, a new way to control carbon source preference

Authors:

Allmann, Stefan and Wargnies, Marion and Cahoreau, Edern and Biran, Marc and Plazolles, Nicolas and Morand, Pauline and Pineda, Erika and Kulyk, Hanna and Asencio, Corinne and Villafraz, Oriana and others,

Journal:

bioRxiv (2019): 800839

Genetic insight into zinc and antimicrobial toxicity uncovers a glycerol phosphate modification on streptococcal rhamnose polysaccharides

Authors:

Edgar, Rebecca J and van Hensbergen, Vincent P and Ruda, Alessandro and Turner, Andrew and Deng, Pan and Le Breton, Yoann and El-Sayed, Najib M and Belew, Ashton T and McIver, Kevin S and McEwan, Alistair G and others,

Journal:

bioRxiv (2018): 337519

References

View all 24 references: Citation Explorer

Interaction of fosfomycin with the glycerol 3-phosphate transporter of Escherichia coli

Authors:

Santoro A, Cappello AR, Madeo M, Martello E, Iacopetta D, Dolce V.

Journal:

Biochim Biophys Acta (2011): 1323

CCAAT/enhancer binding protein-beta negatively regulates the expression of glycerol-3-phosphate dehydrogenase 1 in pig PK-15 cells

Authors:

Gao Y, Pan Y.

Journal:

J Appl Genet (2011): 451

Rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis

Authors:

Frohlich KM, Roberts RA, Housley NA, Audia JP.

Journal:

J Bacteriol (2010): 4281

Effects of salinity changes on the growth of Dunaliella salina and its isozyme activities of glycerol-3-phosphate dehydrogenase

Authors:

Chen H, Jiang JG, Wu GH.

Journal:

J Agric Food Chem (2009): 6178

Design and synthesis of small molecule glycerol 3-phosphate acyltransferase inhibitors

Authors:

Wydysh EA, Medghalchi SM, Vadlamudi A, Townsend CA.

Journal:

J Med Chem (2009): 3317

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B: Enzyme Mix	2 bottles (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Glycerol 3-Phosphate (G3P) Standard	1 vial (lyophilized powder)
Component E: DMSO	1 vial (100 µL)