Amplite® Fluorimetric Glycerol Assay Kit

Amplite® Fluorimetric Glycerol Assay Kit is a fluorimetric assay kit for quantification of glycerol in biological samples.

Detection Principle: Measures H₂O₂ generated from glycerol in an enzyme-coupled reaction.
Sensitivity: Detects glycerol down to 0.015 mg/L (~0.16 µM) in a 100 µL reaction.
Detection Chemistry: Uses Amplite® HRP substrate in HRP-coupled reactions.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.
Plate Format: Recommended for solid black microplates.

Glycerol was measured with Amplite® Fluorimetric Glycerol Assay Kit on a solid black 96-well plate using a Gemini microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13833	200 Tests	Price

Overview

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production. Amplite® Fluorimetric Glycerol Assay Kit offers a sensitive fluorescence-based assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using our Amplite® HRP substrate in HRP-coupled reactions. The fluorescence signal can be measured by a fluorescence microplate reader. With this Fluorimetric Glycerol Assay Kit, we were able to detect as low as 0.015 mg/L (~0.16 µM) glycerol in a 100 µL reaction volume.

References

View all 47 references: Citation Explorer

Rapid monitoring of glycerol in fermentation growth media: Facilitating crude glycerol bioprocess development

Authors:

Abad S, Perez X, Planas A, Turon X.

Journal:

Talanta (2014): 210

Synthesis of substituted 1,3-diesters of glycerol using wittig chemistry

Authors:

Lowe HI, Toyang NJ, Watson CT, Bryant J.

Journal:

Nat Prod Commun (2014): 687

The glycoglycerolipid 1,2-dipalmitoyl-3-(N-palmitoyl-6'-amino-6'-deoxy-alpha-d-glucosyl)-sn-glycerol is no inhibitor of the human Myt1 kinase

Authors:

Rohe A, Gollner C, Erdmann F, Sippl W, Schmidt M.

Journal:

J Enzyme Inhib Med Chem (2014): 514

Functional characterization of Yersinia pestis aerobic glycerol metabolism

Authors:

Willias SP, Chauhan S, Motin VL.

Journal:

Microb Pathog (2014): 33

Quantitative analysis of glycerol levels in human urine by liquid chromatography-tandem mass spectrometry

Authors:

Dong Y, Ma Y, Yan K, Shen L, Wang X, Xu Y, He G, Wu Y, Lu J, Yang Z, Feng F.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2014): 30

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Amplite® HRP Substrate (light sensitive)	1 vial
Component B: Enzyme Mix	2 bottles (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Glycerol Standard	80 µL/vial
Component E: DMSO	1 vial (100 µL)