The Amplite® Fluorimetric Glycogen Assay Kit provides a streamlined assay for the sensitive quantification of glycogen using a reliable two-step enzymatic process. In the first step, glycogen is enzymatically hydrolyzed into glucose. In the second step, the released glucose undergoes an oxidation reaction that produces hydrogen peroxide, which subsequently reacts with the proprietary Amplite® Red fluorogenic dye to generate a red fluorescent signal. The fluorescence intensity, which is directly proportional to the glycogen concentration, can be easily measured using a standard fluorescence microplate reader at Ex/Em of 540/590nm. The kit is compatible with a wide range of biological samples, including cell lysates, tissue extracts, and serum samples. The assay protocol is easy to follow and produces a stable fluorescent signal, allowing glycogen measurements for high-throughput screening or routine analysis in metabolic research, diabetes studies, and other related applications.