Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric HDAC Activity Assay Kit

Green Fluorescence

Amplite® Fluorimetric HDAC Activity Assay Kit Green Fluorescence is a fluorimetric assay kit for detection of histone deacetylase activity in cell lysates, extracts, and purified enzyme preparations.

Detection Principle: Measures HDAC-dependent fluorescence enhancement of HDAC Green™ substrate.
Signal Advantage: Provides a >10-fold higher signal/background ratio than compared peptide-based substrates.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm.
Applications: Supports HDAC activity measurement and in vitro inhibitor screening.

HDAC activity in HeLa nuclear extract was measured with Amplite® Fluorimetric HDAC Activity Assay Kit (in blue) was compared with Vendor X (in red) and Vendor Y (in green), both of which use Ac-RGK(Ac)-R110 peptide substrate. The signal/background ratio of the HDAC activity measured with Amplite® Fluorimetric HDAC Activity Assay Kit is more than 10 times higher than those of Vendors X and Y.

Protocol

SDS

Catalog	Size	Price	Quantity
13601	200 Tests	Price

Overview

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone. Deacetylation restores the positive electric charge of the lysine amino acids, which increases the histone's affinity for the negatively charged phosphate backbone of DNA. This generally down-regulates DNA transcription by blocking the access of transcription factors. HDACs are involved in the pathway by which the retinoblastoma protein (pRb) suppresses cell proliferation. The pRb protein is part of a complex which attracts HDACs to the chromatin so that it will deacetylate histones. HDAC inhibitors are being studied as a treatment for cancer. The Amplite® HDAC Assay Kit provides a quick, convenient, and sensitive method for the detection of HDAC activity. Our HDAC Green™ substrate is much more resistant than other commercial peptide-based HDAC substrates. Our kit can be used for measuring HDAC activity in cell lysates, in vitro inhibitor screening with extracts or purified enzymes. The long wavelength emission and higher extinction coefficient of the HDAC Green™ substrate provide less interference from compounds and cell components. HDAC activity is determined by monitoring the green fluorescence enhancement with excitation at 490 nm and emission at 520 nm.

Citations

View all 27 citations: Citation Explorer

The Marine-Derived Cyclopentapeptide Turnagainolide B Suppresses Melanoma via Autophagic Flux Disruption and Inhibits Tumorigenesis In Vivo

Authors:

Wan, Guoyue and Zhao, Keyu and Wang, Min and Xu, Ren-He and Jin, Meiling and Liu, Liwei

Journal:

Marine Drugs (2026): 235

Gut microbiota-derived butyrate impairs innate antiviral immunity against coronavirus PEDV infection via class IIa HDACs inhibition

Authors:

Yin, Lingdan and Zhu, Qian and Liu, Xiang and Yao, Yao and Ma, Yanlong and Zhang, Guozhong and Zhu, Kui and Liu, Pinghuang

Journal:

Journal of Integrative Agriculture (2026)

Mutant NPM1 modulates PDCD4 ubiquitination degradation and facilitates leukemogenesis

Authors:

Liang, Chuangxuan and Ke, Jing and Zhang, Zhenyu and Guo, Huarong and Shang, Hongxin and Liu, Danwen and Li, Shan and Wu, Fuyun

Journal:

iScience (2025)

The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer

Authors:

Shang, Taiyu and Jiang, Tianyi and Tan, Jiangqi and Jiang, Haolin and Xu, Mengyou and Pan, Yufei and Lin, Yunkai and Cui, Xiaowen and Tian, Chenxi and Feng, Huibo and others,

Journal:

Signal Transduction and Targeted Therapy (2025): 341

Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma

Authors:

Xu, Jun-Yao and Chen, Huan and Yu, Yao-Yu and Chen, Tian-Yi and Wang, Yi-Qiu and Xue, Wei and Zheng, Jun-Hua and Liu, Jin-Yao and Zhai, Wei

Journal:

Cell Reports Medicine (2025)

References

View all 80 references: Citation Explorer

Active site tyrosine is essential for amidohydrolase but not for esterase activity of a class 2 histone deacetylase-like bacterial enzyme

Authors:

Moreth K, Riester D, Hildmann C, Hempel R, Wegener D, Schober A, Schwienhorst A.

Journal:

Biochem J. (2006)

The histone deacetylase inhibitor trichostatin a has genotoxic effects in human lymphoblasts in vitro

Authors:

Olaharski AJ, Ji Z, Woo JY, Lim S, Hubbard AE, Zhang L, Smith MT.

Journal:

Toxicol Sci (2006): 341

Histone deacetylase (HDAC) 4 involvement in both Lewy and Marinesco bodies

Authors:

Takahashi-Fujigasaki J, Fujigasaki H.

Journal:

Neuropathol Appl Neurobiol (2006): 562

Experimental therapy of malignant gliomas using the inhibitor of histone deacetylase MS-275

Authors:

Eyupoglu IY, Hahnen E, Trankle C, Savaskan NE, Siebzehnrubl FA, Buslei R, Lemke D, Wick W, Fahlbusch R, Blumcke I.

Journal:

Mol Cancer Ther (2006): 1248

Role of histone deacetylase Rpd3 in regulating rRNA gene transcription and nucleolar structure in yeast

Authors:

Oakes ML, Siddiqi I, French SL, Vu L, Sato M, Aris JP, Beyer AL, Nomura M.

Journal:

Mol Cell Biol (2006): 3889

Spectral properties

Excitation (nm)	493
Emission (nm)	520

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
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Bulk request	Inquire
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 20, 2026

Component A: HDAC Green™ Substrate	1 vial (40 µL)
Component B: Assay Buffer	1 bottle (40 mL)
Component C: HDAC Inhibitor (Trichostatin A, 3 mM)	1 vial (20 µL)
Component D: Signal Enhancer (50X)	1 vial (200 µL)