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Amplite® Fluorimetric HDAC Activity Assay Kit
Green Fluorescence
Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone. Deacetylation restores the positive electric charge of the lysine amino acids, which increases the histone's affinity for the negatively charged phosphate backbone of DNA. This generally down-regulates DNA transcription by blocking the access of transcription factors. HDACs are involved in the pathway by which the retinoblastoma protein (pRb) suppresses cell proliferation. The pRb protein is part of a complex which attracts HDACs to the chromatin so that it will deacetylate histones. HDAC inhibitors are being studied as a treatment for cancer. The Amplite® HDAC Assay Kit provides a quick, convenient, and sensitive method for the detection of HDAC activity. Our HDAC Green™ substrate is much more resistant than other commercial peptide-based HDAC substrates. Our kit can be used for measuring HDAC activity in cell lysates, in vitro inhibitor screening with extracts or purified enzymes. The long wavelength emission and higher extinction coefficient of the HDAC Green™ substrate provide less interference from compounds and cell components. HDAC activity is determined by monitoring the green fluorescence enhancement with excitation at 490 nm and emission at 520 nm.
HDAC activity in HeLa nuclear extract was measured with Amplite® Fluorimetric HDAC Activity Assay Kit (in blue) was compared with Vendor X (in red) and Vendor Y (in green), both of which use Ac-RGK(Ac)-R110 peptide substrate. The signal/background ratio of the HDAC activity measured with Amplite® Fluorimetric HDAC Activity Assay Kit is more than 10 times higher than those of Vendors X and Y.
HDAC activity in HeLa nuclear extract was measured with Amplite® Fluorimetric HDAC Activity Assay Kit (in blue) was compared with Vendor X (in red) and Vendor Y (in green), both of which use Ac-RGK(Ac)-R110 peptide substrate. The signal/background ratio of the HDAC activity measured with Amplite® Fluorimetric HDAC Activity Assay Kit is more than 10 times higher than those of Vendors X and Y.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
13601200 Tests
Price
 
Spectral properties
Excitation (nm)493
Emission (nm)520
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on October 15, 2025