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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit
Red Fluorescence
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of H2O2 biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. Amplite® Hydrogen Peroxide Assay Kit uses our Amplite® Red peroxidase substrate to quantify hydrogen peroxide in solutions and cell extracts. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized 'mix and read' assay that is compatible with HTS liquid handling instruments.
Hydrogen Peroxide dose response was measured in a solid black 384-well plate with the Amplite® Flourimetric Hydrogen Peroxide Assay Kit using a Gemini fluorescence microplate reader (Molecular Devices).
Hydrogen Peroxide dose response was measured in a solid black 384-well plate with the Amplite® Flourimetric Hydrogen Peroxide Assay Kit using a Gemini fluorescence microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
11501500 Tests
Price
 
Spectral properties

Excitation (nm)571
Emission (nm)584
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 8, 2025