Name: Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Brand: AAT Bioquest
SKU: 13846
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit is a fluorimetric assay kit for quantification of hypochlorite/hypochlorous acid in a microplate format.

Detection Principle: Oxirite™ Hypochlorite Sensor becomes strongly fluorescent upon reaction with hypochlorite.
Sensitivity: Detects hypochlorite down to 0.4 µM in a 100 µL reaction.
Signal Advantage: Provides >100-fold fluorescence enhancement upon hypochlorite detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.

Hypochlorite was measured with Amplite® Fluorimetric Hypochlorite/Hypochlorous Acid Assay Kit in a 96-well solid black plate.

Protocol

SDS

Catalog	Size	Price	Quantity
13846	200 Tests	Price

Overview

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance. Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit offers a sensitive fluorescence-based assay for measuring hypochlorite (hypochlorous acid) with high specificity. Upon selective reaction with hypochlorite (hypochlorous) the weakly fluorescent Oxirite™ Hypochlorite Sensor generates a strongly fluorescent product that gives more than 100-fold fluorescence enhancement. The fluorescence signal can be measured by a fluorescence microplate reader. With this Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit, as low as 0.4 µM hypochlorite was detected in a 100 µL reaction volume.

Citations

View all 3 citations: Citation Explorer

Myeloperoxidase and its derivative hypochlorous acid combined clinical indicators predict new-onset atrial fibrillation in sepsis: a case-control study

Authors:

Dai, Hui and Ye, Jiawei and Wang, Shangyuan and Li, Xingyao and Li, Wenjie

Journal:

BMC Cardiovascular Disorders (2024): 1--12

Artificial Neutrophils Against Vascular Graft Infection

Authors:

Jiang, Wentao and Xu, Huizi and Gao, Zheng and Wu, Ziyu and Zhao, Zichun and Wang, Jun and Wu, Yawen and Ke, Haifeng and Mao, Chun and Wan, Mimi and others,

Journal:

Advanced Science (2024): 2402768

Antioxidant microspheres as drug delivery vehicles for the prevention of post-traumatic osteoarthritis

Authors:

Kavanaugh, Taylor Elizabeth

References

View all 60 references: Citation Explorer

Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation

Authors:

Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.

Journal:

J Endod (2014): 51

Effect of hypochlorite oxidation on cholinesterase-inhibition assay of acetonitrile extracts from fruits and vegetables for monitoring traces of organophosphate pesticides

Authors:

Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.

Journal:

J Toxicol Sci (2014): 71

A simple yet effective chromogenic reagent for the rapid estimation of bromate and hypochlorite in drinking water

Authors:

Zhang J, Yang X.

Journal:

Analyst (2013): 434

Use of pyrogallol red and pyranine as probes to evaluate antioxidant capacities towards hypochlorite

Authors:

Perez-Cruz F, Cortes C, Atala E, Bohle P, Valenzuela F, Olea-Azar C, Speisky H, Aspee A, Lissi E, Lopez-Alarcon C, Bridi R.

Journal:

Molecules (2013): 1638

Effect of hypochlorite-based disinfectants on inactivation of murine norovirus and attempt to eliminate or prevent infection in mice by addition to drinking water

Authors:

Takimoto K, Taharaguchi M, Sakai K, Takagi H, Tohya Y, Yamada YK.

Journal:

Exp Anim (2013): 237

Spectral properties

Excitation (nm)	555
Emission (nm)	576

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
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Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Oxirite™ Hypochlorite Sensor (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Hypochlorite Standard	1 vial (1M, 300 µL)
Component D: DMSO	1 vial (100 µL)