Amplite® Fluorimetric L-Alanine Assay Kit

Amplite® Fluorimetric L-Alanine Assay Kit is a fluorimetric assay kit for quantification of L-alanine in biological samples.

Detection Principle: Uses an enzyme-coupled reaction that generates hydrogen peroxide.
Detection Chemistry: Uses Quest Fluor™ L-Alanine Sensor for fluorescent detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.

L-alanine dose response was measured with Amplite® Fluorimetric L-Alanine Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13825	200 Tests	Price

Overview

L-alanine (L-Ala) plays a crucial role as a building block of important proteins. L-alanine is mostly synthesized by the muscle cells from lactic acid and absorbed into blood via the liver. It is converted into pyruvate by glutamic-pyruvic transaminase to enter the metabolic mainstream. L-Ala is critical for the production of glucose and hence blood sugar management, and plays an important role in the immune system and prevention of kidney stones. Insufficiency of L-alanine is usually a sign of poor nutrition, low protein diet as well as stress. AAT Bioquest's Amplite® Fluorimetric L-Alanine Assay Kit offers a sensitive fluorescent assay for quantifying L-alanine in biological samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which is detected by Quest Fluor™ L-Alanine Sensor with a fluorescence microplate reader.

Citations

View all 2 citations: Citation Explorer

The catabolism of alanine and glutamate ensure proper sporulation by respectively preventing premature germination and providing energy sources

Authors:

Lyu, Fengzhi and Zhang, Tianyu and Yang, Dong and Gui, Meng and Wang, Yongtao and Liao, Xiaojun and Rao, Lei

Journal:

bioRxiv (2024): 2024--03

Colorectal Cancer Progression Is Potently Reduced by a Glucose-Free, High-Protein Diet: Comparison to Anti-EGFR Therapy. Cancers 2021, 13, 5817

Authors:

Skibbe, K and Brethack, AK and S{\"u}nderhauf, A and Ragab, M and Raschdorf, A and Hicken, M and Schlichting, H and Preira, J and Brandt, J and Castven, D and others,

References

View all 39 references: Citation Explorer

Reactivity of beta-methylamino-L-alanine in complex sample matrixes complicating detection and quantification by mass spectrometry

Authors:

Glover WB, Liberto CM, McNeil WS, Banack SA, Shipley PR, Murch SJ.

Journal:

Anal Chem (2012): 7946

Synthesis and characterization of silver/alanine nanocomposites for radiation detection in medical applications: the influence of particle size on the detection properties

Authors:

Guidelli EJ, Ramos AP, Zaniquelli ME, Nicolucci P, Baffa O.

Journal:

Nanoscale (2012): 2884

Molecular beacon based bioassay for highly sensitive and selective detection of nicotinamide adenine dinucleotide and the activity of alanine aminotransferase

Authors:

Tang Z, Liu P, Ma C, Yang X, Wang K, Tan W, Lv X.

Journal:

Anal Chem (2011): 2505

Detection of farnesyltransferase interface hot spots through computational alanine scanning mutagenesis

Authors:

Perez MA, Sousa SF, Oliveira EF, Fern and es PA, Ramos MJ.

Journal:

J Phys Chem B (2011): 15339

Rapid detection of alanine aminotransferase with near-infrared spectroscopy

Authors:

Huang FR, Zhang J, Luo YH, Li SP, Zheng SF, Chen XD.

Journal:

Guang Pu Xue Yu Guang Pu Fen Xi (2010): 2620

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Quest Fluor™ L-Alanine Sensor	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: L-Alanine Standard	1 vial (100 mM, 100 µL)
Component E: DMSO	1 vial (100 µL)