Amplite® Fluorimetric L-Alanine Assay Kit

L-alanine (L-Ala) plays a crucial role as a building block of important proteins. L-alanine is mostly synthesized by the muscle cells from lactic acid and absorbed into blood via the liver. It is converted into pyruvate by glutamic-pyruvic transaminase to enter the metabolic mainstream. L-Ala is critical for the production of glucose and hence blood sugar management, and plays an important role in the immune system and prevention of kidney stones. Insufficiency of L-alanine is usually a sign of poor nutrition, low protein diet as well as stress. AAT Bioquest's Amplite® Fluorimetric L-Alanine Assay Kit offers a sensitive fluorescent assay for quantifying L-alanine in biological samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which is detected by Quest Fluor™ L-Alanine Sensor with a fluorescence microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13825	200 Tests	Price

Citations

View all 2 citations: Citation Explorer

The catabolism of alanine and glutamate ensure proper sporulation by respectively preventing premature germination and providing energy sources

Authors:

Lyu, Fengzhi and Zhang, Tianyu and Yang, Dong and Gui, Meng and Wang, Yongtao and Liao, Xiaojun and Rao, Lei

Journal:

bioRxiv (2024): 2024--03

Colorectal Cancer Progression Is Potently Reduced by a Glucose-Free, High-Protein Diet: Comparison to Anti-EGFR Therapy. Cancers 2021, 13, 5817

Authors:

Skibbe, K and Brethack, AK and S{\"u}nderhauf, A and Ragab, M and Raschdorf, A and Hicken, M and Schlichting, H and Preira, J and Brandt, J and Castven, D and others,

References

View all 39 references: Citation Explorer

Reactivity of beta-methylamino-L-alanine in complex sample matrixes complicating detection and quantification by mass spectrometry

Authors:

Glover WB, Liberto CM, McNeil WS, Banack SA, Shipley PR, Murch SJ.

Journal:

Anal Chem (2012): 7946

Synthesis and characterization of silver/alanine nanocomposites for radiation detection in medical applications: the influence of particle size on the detection properties

Authors:

Guidelli EJ, Ramos AP, Zaniquelli ME, Nicolucci P, Baffa O.

Journal:

Nanoscale (2012): 2884

Molecular beacon based bioassay for highly sensitive and selective detection of nicotinamide adenine dinucleotide and the activity of alanine aminotransferase

Authors:

Tang Z, Liu P, Ma C, Yang X, Wang K, Tan W, Lv X.

Journal:

Anal Chem (2011): 2505

Detection of farnesyltransferase interface hot spots through computational alanine scanning mutagenesis

Authors:

Perez MA, Sousa SF, Oliveira EF, Fern and es PA, Ramos MJ.

Journal:

J Phys Chem B (2011): 15339

Rapid detection of alanine aminotransferase with near-infrared spectroscopy

Authors:

Huang FR, Zhang J, Luo YH, Li SP, Zheng SF, Chen XD.

Journal:

Guang Pu Xue Yu Guang Pu Fen Xi (2010): 2620

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on July 17, 2026

Component A: Quest Fluor™ L-Alanine Sensor	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: L-Alanine Standard	1 vial (100 mM, 100 µL)
Component E: DMSO	1 vial (100 µL)