Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit is a fluorimetric assay kit for quantification of aspartate in biological samples.

Detection Principle: Converts aspartate to pyruvate, generating H₂O₂ through an enzyme-coupled reaction.
Detection Chemistry: Uses Amplite® Red substrate to measure generated H₂O₂. AAT Bioquest
Sensitivity: Quantifies aspartate across a 0.1–100 µM standard range.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm in a microplate reader.

Aspartate dose response was measured with Amplite® Fluorimetric Aspartate Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13827	200 Tests	Price

Overview

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid. Aspartate is involved in the control point of pyrimidine biosynthesis, in transamination reactions, interconversions with asparagine, in the metabolic pathway leading to AMP, in the urea cycle, and is a precursor to homoserine, threonine, isoleucine, and methionine. It is also involved in the malate aspartate shuttle. Amplite® Fluorimetric Aspartate Assay Kit offers a sensitive fluorescent assay for quantifying aspartate in biological samples. Aspartate is converted to pyruvate that generates hydrogen peroxide through an enzyme coupled reaction. The amount of hydrogen peroxide generated by aspartate is monitored with Amplite® Red substrate for quantifying aspartate by a fluorescence microplate reader.

Citations

View all 2 citations: Citation Explorer

Metabolic engineering of Streptomyces roseosporus for increased production of clinically important antibiotic daptomycin

Authors:

Li, Xingwang and Sang, Ziwei and Zhao, Xuejin and Wen, Ying

Journal:

Microbial Biotechnology (2024): e70038

Experimentally evolved Staphylococcus aureus shows increased survival in the presence of Pseudomonas aeruginosa by acquiring mutations in the amino acid transporter, GltT

Authors:

Alexander, Ashley M and Luu, Justin M and Raghuram, Vishnu and Bottacin, Giulia and van Vliet, Simon and Read, Timothy D and Goldberg, Joanna B

Journal:

Microbiology (2024): 001445

References

View all 20 references: Citation Explorer

A systematic and mechanistic evaluation of aspartic acid as filler for directly compressed tablets containing trimethoprim and trimethoprim aspartate

Authors:

ElShaer A, Hanson P, Mohammed AR.

Journal:

Eur J Pharm Biopharm (2013): 468

Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase

Authors:

Kato S, Ikuta T, Hemmi H, Takahashi S, Kera Y, Yoshimura T.

Journal:

Biosci Biotechnol Biochem (2012): 2150

Distribution of free D-aspartic acid and D-aspartate oxidase in frog Rana esculenta tissues

Authors:

Di Giovanni M, Burrone L, Chieffi Baccari G, Topo E, Santillo A.

Journal:

J Exp Zool A Ecol Genet Physiol (2010): 137

Thyroid hormones and D-aspartic acid, D-aspartate oxidase, D-aspartate racemase, H2O2, and ROS in rats and mice

Authors:

Topo E, Fisher G, Sorricelli A, Errico F, Usiello A, D'Aniello A.

Journal:

Chem Biodivers (2010): 1467

Aslfm, the D-aspartate ligase responsible for the addition of D-aspartic acid onto the peptidoglycan precursor of Enterococcus faecium

Authors:

Bellais S, Arthur M, Dubost L, Hugonnet JE, Gutmann L, van Heijenoort J, Legr and R, Brouard JP, Rice L, Mainardi JL.

Journal:

J Biol Chem (2006): 11586

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Conversion Mix	2 vials (lyophilized powder)
Component E: Aspartate Standard	1 vial (lyophilized powder)
Component F: DMSO	1 vial (100 µL)