Name: Amplite® Fluorimetric L-Lactate Assay Kit
Brand: AAT Bioquest
SKU: 13814
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric L-Lactate Assay Kit

Amplite® Fluorimetric L-Lactate Assay Kit is a fluorimetric assay kit for quantification of L-lactate in biological samples including serum, plasma, urine, and cell culture samples.

Detection Principle: Measures L-lactate through an enzyme-coupled reaction that generates NADH.
Sensitivity: Detects as little as 1.4 µM L-lactate in a 100 µL reaction.
Detection Chemistry: Uses a fluorogenic NADH sensor for signal generation.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.

L-lactate dose response was measured with Amplite® Fluorimetric L-Lactate Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13814	200 Tests	Price

Overview

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid. Lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in the process of metabolism and exercise. Monitoring lactate levels is a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is not metabolized by mammals and its elimination from the body depends mainly on renal excretion. D- and L-lactic acid are found in many fermented milk products such as yoghurt and cheese, and also in pickled vegetables, and cured meats and fish. The D- and L-lactic acid (generated by bacteria) is a quality indicator of foods, such as egg, milk, fruit juice and wine. Abnormal high concentration of D-lactate in the blood is usually a reflection of bacterial overgrowth in the gastrointestinal tract. AAT Bioquest's Amplite® Lactate Assay Kits (Cat# 13814 and 13815 for L-lactate assay, and Cat# 13810 and 13811 for D-lactate assay) provide both fluorescence and absorbance-based method for detecting either L-lactate or D-lactate in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, lactate is proportionally related to NADH, which is specifically monitored by a fluorogenic NADH sensor. The signal can be read by a fluorescence microplate reader. With this Fluorimetric Amplite® L-Lactate Assay Kit, we were able to detect as little as 1.4 µM L-lactate in a 100 µL reaction volume.

Citations

View all 26 citations: Citation Explorer

ROS-driven mitochondrial and mtDNA release as a novel pathway of metabolic injury in diabetes

Authors:

Chen, Dandan and Jia, Yuxin and Duan, Xiaohong and Huang, Jianhua and Deng, Xiaohong

Journal:

Journal of Molecular Endocrinology (2026): JME--25

A novel m. 5906G> a variant in MT-CO1 causes MELAS/Leigh overlap syndrome

Authors:

Liu, Zhimei and Xie, Yaojun and Lou, Xiaoting and Zeng, Xiaofei and Zhang, Luyi and Yu, Meng and Wang, Junling and Li, Jiuwei and Shen, Danmin and Li, Hua and others,

Journal:

Molecular Genetics and Genomics (2024): 1--14

TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth

Authors:

Xia, Tian and Meng, Lian and Xu, Guixuan and Sun, Hao and Chen, Hao

Journal:

Cell Death \& Disease (2024): 740

De novo frameshift variant in MT-ND1 causes a mitochondrial complex I deficiency associated with MELAS syndrome

Authors:

Lou, Xiaoting and Zhou, Yuwei and Liu, Zhimei and Xie, Yaojun and Zhang, Luyi and Zhao, Suzhou and Gong, Shuai and Zhuo, Xiuwei and Wang, Junling and Dai, Lifang and others,

Journal:

Gene (2023): 147229

METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression

Authors:

Xia, Peng and Zhang, Hao and Lu, Haofeng and Xu, Kequan and Jiang, Xiang and Jiang, Yuke and Gongye, Xiangdong and Chen, Zhang and Liu, Jie and Chen, Xi and others,

Journal:

Cancer Communications (2023)

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Serum lactate dehydrogenase predicts for overall survival benefit in patients with metastatic renal cell carcinoma treated with inhibition of mammalian target of rapamycin

Authors:

Armstrong AJ, George DJ, Halabi S.

Journal:

J Clin Oncol (2012): 3402

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Enzyme Mix	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: L-Lactate Standard	2.25 mg/vial