Name: Amplite® Fluorimetric L-Lactate Assay Kit
Brand: AAT Bioquest
SKU: 13814
Price: 348 USD
Availability: InStock

Amplite® Fluorimetric L-Lactate Assay Kit

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid. Lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in the process of metabolism and exercise. Monitoring lactate levels is a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is not metabolized by mammals and its elimination from the body depends mainly on renal excretion. D- and L-lactic acid are found in many fermented milk products such as yoghurt and cheese, and also in pickled vegetables, and cured meats and fish. The D- and L-lactic acid (generated by bacteria) is a quality indicator of foods, such as egg, milk, fruit juice and wine. Abnormal high concentration of D-lactate in the blood is usually a reflection of bacterial overgrowth in the gastrointestinal tract. AAT Bioquest's Amplite® Lactate Assay Kits (Cat# 13814 and 13815 for L-lactate assay, and Cat# 13810 and 13811 for D-lactate assay) provide both fluorescence and absorbance-based method for detecting either L-lactate or D-lactate in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, lactate is proportionally related to NADH, which is specifically monitored by a fluorogenic NADH sensor. The signal can be read by a fluorescence microplate reader. With this Fluorimetric Amplite® L-Lactate Assay Kit, we were able to detect as little as 1.4 µM L-lactate in a 100 µL reaction volume.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13814	200 Tests	Price

Citations

View all 25 citations: Citation Explorer

A novel m. 5906G> a variant in MT-CO1 causes MELAS/Leigh overlap syndrome

Authors:

Liu, Zhimei and Xie, Yaojun and Lou, Xiaoting and Zeng, Xiaofei and Zhang, Luyi and Yu, Meng and Wang, Junling and Li, Jiuwei and Shen, Danmin and Li, Hua and others,

Journal:

Molecular Genetics and Genomics (2024): 1--14

TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth

Authors:

Xia, Tian and Meng, Lian and Xu, Guixuan and Sun, Hao and Chen, Hao

Journal:

Cell Death \& Disease (2024): 740

De novo frameshift variant in MT-ND1 causes a mitochondrial complex I deficiency associated with MELAS syndrome

Authors:

Lou, Xiaoting and Zhou, Yuwei and Liu, Zhimei and Xie, Yaojun and Zhang, Luyi and Zhao, Suzhou and Gong, Shuai and Zhuo, Xiuwei and Wang, Junling and Dai, Lifang and others,

Journal:

Gene (2023): 147229

METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression

Authors:

Xia, Peng and Zhang, Hao and Lu, Haofeng and Xu, Kequan and Jiang, Xiang and Jiang, Yuke and Gongye, Xiangdong and Chen, Zhang and Liu, Jie and Chen, Xi and others,

Journal:

Cancer Communications (2023)

N-glycosylation stabilizes MerTK and promotes hepatocellular carcinoma tumor growth

Authors:

Liu, Yongzhang and Lan, Linhua and Li, Yujie and Lu, Jing and He, Lipeng and Deng, Yao and Fei, Mingming and Lu, Jun-Wan and Shangguan, Fugen and Lu, Ju-Ping and others,

Journal:

Redox biology (2022): 102366

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Serum lactate dehydrogenase predicts for overall survival benefit in patients with metastatic renal cell carcinoma treated with inhibition of mammalian target of rapamycin

Authors:

Armstrong AJ, George DJ, Halabi S.

Journal:

J Clin Oncol (2012): 3402

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Fax
Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 10, 2025

Component A: Enzyme Mix	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: L-Lactate Standard	2.25 mg/vial