Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Amplite® Fluorimetric Lead (II) Ion Quantitation Kit is a fluorimetric assay kit for quantification of lead (II) ions in solution.

Detection Principle: Uses Lead Green™, a fluorescence probe that responds to Pb²⁺.
Sensitivity: Detects lead (II) ions down to 4 µM.
Workflow: No separation steps required; assay can be completed within 30 minutes.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/530 nm in 96- or 384-well microplates.

Lead dose response was measured with Amplite® Fluorimetric Lead (II) Ion Quantitation Kit in a 96-well solid black plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
19007	200 Tests	Price

Overview

Lead is a highly poisonous metal to human beings, especially young children. Lead interferes with cell signaling and gene expression, causing severe damages to the brain, liver, kidney, and even death. Because of its long history in mining and smelting, as well as widespread use in battery, paint and gasoline, lead pollution in soil and groundwater has been one of the most serious environmental problems. Amplite® Fluorimetric Lead (II) Ion Quantitation Kit provides a robust method for detecting lead (II) ion in solution. It uses Lead Green™, a selective and sensitive green fluorescence probe that can be easily monitored with a fluorescence microplate reader (Ex/Em = 490/530 nm). The Amplite® Fluorimetric Lead (II) Ion Quantitation Kit can be performed in a convenient 96-well or 384-well microplate format and easily adapted to automation with no separation steps required. The assay can be completed within 30 minutes. With the Amplite® Fluorimetric Lead (II) Ion Quantitation Kit, as little as 4µM lead (II) ion was detected.

Citations

View all 20 citations: Citation Explorer

Lead toxicity from retained bullet fragments: A systematic review and meta-analysis

Authors:

Apte, A., Bradford, K., Dente, C., Smith, R. N.

Journal:

J Trauma Acute Care Surg (2019): 707-716

Toxicodynamics of Lead, Cadmium, Mercury and Arsenic- induced kidney toxicity and treatment strategy: A mini review

Authors:

Rana, M. N., Tangpong, J., Rahman, M. M.

Journal:

Toxicol Rep (2018): 704-713

A critical review on speciation, mobilization and toxicity of lead in soil-microbe-plant system and bioremediation strategies

Authors:

Kushwaha, A., Hans, N., Kumar, S., Rani, R.

Journal:

Ecotoxicol Environ Saf (2018): 1035-1045

Severe Systemic Lead Toxicity Resulting From Extra-Articular Retained Shrapnel Presenting as Jaundice and Hepatitis: A Case Report and Review of the Literature

Authors:

Grasso, I. A., Blattner, M. R., Short, T., Downs, J. W.

Journal:

Mil Med (2017): e1843-e1848

Retained Lumbar Bullet: A Case Report of Chronic Lead Toxicity and Review of the Literature

Authors:

Bustamante, N. D., Macias-Konstantopoulos, W. L.

Journal:

J Emerg Med (2016): 45-9

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	530 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 23, 2026

Component A: Lead Green	1 vial
Component B: Lead (II) Assay Buffer	1 bottle (10 mL)
Component C: Lead Standard (100 mM)	1 vial (0.2 mL)
Component D: DMSO	1 vial (100 uL)