Amplite® Fluorimetric Lipase Activity Assay Kit

Amplite® Fluorimetric Lipase Activity Assay Kit is a fluorimetric assay kit for quantification of lipase activity in cells, supernatants, tissue extracts, and serum.

Detection Principle: Uses a coupled enzymatic reaction that converts a lipase substrate into a fluorescent product.
Signal Response: Fluorescence intensity is proportional to lipase activity in the sample.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/610 nm.
Applications: Suitable for biological samples and adaptable to high-throughput screening.

Lipase dose response was measured with the Amplite® Fluorimetric Lipase Activity Assay Kit on a 96-well clear bottom black solid microplate using a fluorescence microplate reader (Ex/Em = 540/610, Cutoff = 570 nm).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11322	100 Tests	Price

Overview

The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity. This assay leverages a coupled enzymatic reaction that converts a substrate into a fluorescent product (Ex/Em=540/610 nm), with fluorescence intensity proportional to the lipase activity present in the sample. This kit is suitable for detecting lipases from various biological samples like cells, supernatants, tissue extracts, or serum, and is adaptable for high-throughput screening applications. Lipases belong to a class of enzymes that catalyze the hydrolysis of ester bonds in lipids, such as triglycerides, phospholipids, and cholesterol esters. These enzymes are essential for the digestion, absorption, and metabolism of dietary fats in the body, playing crucial a role in lipid metabolism and cell signaling. Lipases are produced by various organs and tissues, including the pancreas, liver, intestine, and adipose tissue. Accurate measurement of lipase activity is critical in screening and diagnosis of diseases like pancreatitis, cystic fibrosis, celiac disease, Crohn's disease, and hyperlipidemia.

References

View all 50 references: Citation Explorer

The effect of konjac glucomannan on enzyme kinetics and fluorescence spectrometry of digestive enzymes: An in vitro research from the perspective of macromolecule crowding.

Authors:

Chen, Wenjing and Li, Sha and Albahi, Amgad and Ye, Shuxin and Li, Jing and Li, Bin

Journal:

Food research international (Ottawa, Ont.) (2024): 114247

Green-emitting carbon dots as a "turn on" fluorescence bio-probe for highly sensitive and selective detection of lipase in human serum.

Authors:

Al-Mashriqi, Haitham Saad and Sanga, Pascaline and Chen, Jia and Li, Xin and Xiao, Jing and Li, Yan and Qiu, Hongdeng

Journal:

Analytical and bioanalytical chemistry (2024): 971-981

A pH-Sensitive Double Chromophore Fluorescent Dye for Live-Tracking of Lipophagy.

Authors:

Engelhardt, Pascal M and Veronese, Matteo and Eryiğit, Alpay A and Das, Anushka and Kaczmarek, Alexander T and Rugarli, Elena I and Schmalz, Hans-Günther

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2024): e202400808

Bioconversion of waste glycerol into viscosinamide by Pseudomonas fluorescens DR54 and its activity evaluation.

Authors:

Jama, Dominika and Łaba, Wojciech and Kruszelnicki, Mateusz and Polowczyk, Izabela and Lazar, Zbigniew and Janek, Tomasz

Journal:

Scientific reports (2024): 1531

A turn-on fluorescence sensor for detection of heparinase with heparin templated aggregation of tetracationic porphyrin derivative.

Authors:

Pandey, Shrishti P and Singh, Prabhat K and Jha, Pamela and Jobby, Renitta

Journal:

International journal of biological macromolecules (2023): 125934

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	610 nm
Cutoff	570 nm
Recommended plate	Solid black, or black plate with clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 25, 2026

Component A: Lipase Assay Buffer	25 mL
Component B: Lipase Substrate Buffer	10 mL
Component C: Lipase Substrate	0.25 mL
Component D: Lipase Standard	1 Vial