The Amplite® Fluorimetric Lipoxygenase Activity Assay kit offers a robust, convenient and rapid method for measuring lipoxygenase (LOX) activity in biological samples. Lipoxygenases are iron-containing oxidoreductase enzymes that are involved in the dioxygenation of unsaturated fatty acids, such as arachidonic acid and linoleic acid, to produce hydroperoxides. These hydroperoxides serve as intermediates for the production of leukotrienes and lipoxins, which are signaling molecules that regulate inflammatory responses involved in inflammation, cancer, and other biological processes. Our Amplite® Fluorimetric Lipoxygenase Activity Assay kit detects lipoxygenases based on a fluorescence detection system. The substrate is oxidized by lipoxygenase present in the sample, producing a fluorigenic compound resulting in an enhanced fluorescence signal with Ex/Em=490/530nm. The generated signal is directly proportional to the enzymatic activity, enabling accurate quantification of the enzyme. This kit provides a simple, reliable solution for detecting lipoxygenase activity in various biological samples, including cell lysates, serum, purified enzymes, and tissue homogenates. It is compatible with fluorescence microplate readers and is ideal for studying enzyme kinetics, screening inhibitors, or investigating disease-related pathways.