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Amplite® Fluorimetric Lithium Ion Quantification Kit

Quantifying lithium ions is important in various scientific fields and industries, including biochemistry, medicine, environmental analysis, and food science, etc. The rapid and accurate determination of lithium ions is particularly important in the battery industry. There are several methods commonly used to quantify lithium ions, including flame photometry, ion-selective electrodes (ISE), atomic absorption spectroscopy, and fluorescence spectrophotometry. Flame photometry and atomic absorption spectroscopy require the inflammation of the samples. They are tedious to use and require expensive and sophisticated instrumentation. Ion-selective electrodes require large volumes of samples and often have low selectivity. Among all the methods, fluorescence spectrophotometry is the most convenient method for quantifying lithium ions. Fluorescence spectrophotometry involves complexing lithium ions with specific reagents and measuring the resulting fluorescence changes. However, there is still lacking a fluorescence-based lithium ion assay kit in the commercial market due to the absence of a robust fluorescence lithium ion indicator. Amplite® Fluorimetric Lithium Ion Quantification Kit uses our new robust lithium-ion indicator dye, Lithiumighty™ 520, which exhibits great fluorescence intensity enhancement upon binding to lithium ions. Lithiumighty ™ 520 is perhaps the most robust lithium-ion indicator with high selectivity. It enables the kit to be useful for the rapid determination of lithium concentrations in a variety of samples compared to the other commercial lithium-ion assays. This microplate-based assay kit requires an extremely small amount of sample. It is particularly suitable for the determination of lithium ion concentration in a microvolume format.

Lithium dose response was measured with Amplite® Fluorimetric Lithium Ion Kit in a 96-well solid black plate.
Lithium dose response was measured with Amplite® Fluorimetric Lithium Ion Kit in a 96-well solid black plate.
CatalogSize
Price
Quantity
21351100 Tests
Price
 
Spectral properties

Excitation (nm)491
Emission (nm)513
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Instrument settings

Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on October 14, 2025