Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Maleimide Quantitation Kit

Green Fluorescence

Detection Principle: Uses Maleimide Green™ fluorescence enhancement upon reaction with maleimide groups.
Sensitivity: Detects as little as 10 pmol of maleimide (100 nM) in a 100 µL assay.
Workflow: One-step assay compatible with 96- or 384-well microplates and can be adapted to automation.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm.

N-ethylmaleimide dose response was measured in a 96-well solid black
plate with Amplite™ Fluorimetric Maleimide Quantitation Assay Kit using a
NOVOstar microplate reader (BMG Labtech).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5523	200 Tests	Price

Overview

A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules. There are few reagents or assay kits available for quantitate the number of maleimide groups that are introduced into the first protein. All the commercial kits have tedious protocols. Our kit uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorimetric method to detect as little as 10 picomole of maleimide in a 100 µL assay volume (100 nM in concentration). The assay is rapid and robust. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The kit provides a convenient protocol with all the essential reagents. For the rapid quantification of a protein maleimide group we recommend you use #5526. For the quantification of a nano particle maleimide group we recommend you use #5525.

Citations

View all 11 citations: Citation Explorer

Regulation of pancreatic cancer microenvironment by an intelligent gemcitabine@ nanogel system via in vitro 3D model for promoting therapeutic efficiency

Authors:

Chen, Di and Zhu, Xiaofei and Tao, Wanru and Kong, Yan and Huag, Yong and Zhang, Yajun and Liu, Ri and Jiang, Lingong and Tang, Ying and Yu, Haiyan and others,

Journal:

Journal of Controlled Release (2020)

Prote{\'\i}nas fluorescentes como sensibilizadores de emiss{\~a}o em nanopart{\'\i}culas contendo Eu3+

Authors:

Maturi, Fernando Eduardo

Restricting the conformational freedom of the neuronal nitric-oxide synthase flavoprotein domain reveals impact on electron transfer and catalysis

Authors:

Dai, Yue and Haque, Mohammad Mahfuzul and Stuehr, Dennis J

Journal:

Journal of Biological Chemistry (2017): 6753--6764

Study of Electron Transfer through the Reductase Domain of Neuronal Nitric Oxide Synthase and Development of Bacterial Nitric Oxide Synthase Inhibitors

Authors:

Dai, Yue

Conjugation with an inulin-chitosan adjuvant markedly improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10. 4 fusion protein

Authors:

Yu, Weili and Hu, Tao

Journal:

Molecular Pharmaceutics (2016)

References

View all 38 references: Citation Explorer

A new maleimide-bound acid-cleavable solid-support reagent for profiling phosphorylation

Authors:

Chowdhury SM, Munske GR, Siems WF, Bruce JE.

Journal:

Rapid Commun Mass Spectrom (2005): 899

Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo3

Authors:

Chen J, Lu Z, Lawrence TS, Smith DE.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2005): 161

Quantitation of microparticles released from coated-platelets

Authors:

Dale GL, Remenyi G, Friese P.

Journal:

J Thromb Haemost (2005): 2081

Characterization of site-specific ScFv PEGylation for tumor-targeting pharmaceuticals

Authors:

Natarajan A, Xiong CY, Albrecht H, DeNardo GL, DeNardo SJ.

Journal:

Bioconjug Chem (2005): 113

Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides

Authors:

Li J, Xu Q, Cortes DM, Perozo E, Laskey A, Karlin A.

Journal:

Proc Natl Acad Sci U S A (2002): 11605

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Maleimide Green™	1 vial
Component B: Reaction Buffer	1 vial (500 μL)
Component C: Assay Buffer	1 bottle (25 mL)
Component D: N-ethylmaleimide Standard	1 vial (10 mM, 50 μL )
Component E: DMSO	1 vial (200 μL)