Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit

Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit is a fluorimetric assay kit for quantification of malondialdehyde, a biomarker of lipid peroxidation and oxidative stress.

Detection Principle: Monoaldelite™ Blue generates strong fluorescence upon reaction with MDA.
Workflow: Measures MDA without the heating steps required by conventional TBARS-based assays.
Instrument Compatibility: Fluorescence detection at Ex/Em 365/435 nm with a microplate reader.
Applications: Supports assessment of lipid peroxidation and oxidative stress.

MDA dose response was measured with Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit on a 96-well solid black microplate using a Gemini microplate reader (Molecular Devices) at Ex/Em=365/435 nm, cutoff=420 nm.

Protocol

SDS

Catalog	Size	Price	Quantity
10071	200 Tests	Price

Overview

Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are natural byproducts of lipid peroxidation. As the most popular and reliable biomarker for lipid peroxidation, MDA has been widely used for many years to determine oxidative stress in clinical situations. Therefore, quantification of MDA is essential to assess oxidative stress in pathophysiological processes. The Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit offers a quick and convenient method to measure MDA without heating steps that are required for the commercial MDA assay kits from other vendors. Monoaldelite™ Blue itself is nearly non-fluorescent, but generates strong blue fluorescence upon reacting with MDA.

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	365 nm
Emission	435 nm
Cutoff	420nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Monoaldelite™ Blue	2 vials (lyophilized powder)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: MDA Standard	1 vial (lyophilized powder)
Component D: Reaction Solution	1 bottle (5 mL)
Component E: DMSO	1 bottle (100 µL)