Name: Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit *Enhanced Selectivity*
Brand: AAT Bioquest
SKU: 10072
Price: 475 USD
Availability: InStock

Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit

Enhanced Selectivity

Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit *Enhanced Selectivity* is a fluorimetric assay kit for quantification of malondialdehyde, a biomarker of lipid peroxidation and oxidative stress.

Detection Principle: MDA Green™ generates enhanced green fluorescence upon reaction with MDA.
Specificity: Selective for MDA with little interference from other aldehydes.
Workflow: Measures MDA without the heating steps required by TBARS-based assays.
Instrument Compatibility: Fluorescence detection at Ex/Em 480/555 nm with a microplate reader.

MDA dose response was measured with Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit on a 96-well solid black microplate using a Gemini microplate reader (Molecular Devices) at Ex/Em=480/555 nm, cutoff=530 nm.

Protocol

SDS

Catalog	Size	Price	Quantity
10072	200 Tests	Price

Overview

Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation. It is widely used as a reliable biomarker to determine oxidative stress, and the quantification of MDA is an essential way to assess oxidative stress in pathophysiological processes. Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit offers a new method to measure MDA without the heating steps required for the TBARS-based MDA assay. The MDA Green™ can react with MDA to generate a considerable enhancement of green fluorescence signal in a convenient 96-well or 384-well microtiter-plate format. Unlike other commercial MDA assay kits, this assay is robust and specific to MDA with little interference from other aldehydes.

Citations

View all 92 citations: Citation Explorer

The Evaluation of Glutathione Reductase and Malondialdehyde Levels in Patients With Lumbar Disc Degeneration Disease

Authors:

Bakirezer, S. D., Yaltirik, C. K., Kaya, A. H., Yilmaz, S. G., Ozdogan, S., Billur, D., Isbir, T.

Journal:

In Vivo (2019): 811-814

Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria

Authors:

Sharif Usman, S., Dahiru, M., Abdullahi, B., Abdullahi, S. B., Maigari, U. M., Ibrahim Uba, A.

Journal:

Heliyon (2019): e02214

Malondialdehyde Assay in the Evaluation of Aspirin Antiplatelet Effects

Authors:

Polzin, A., Dannenberg, L., Schneider, T., Knoop, B., Naguib, D., Helten, C., Pohl, M., Kelm, M., Zeus, T., Hohlfeld, T.

Journal:

Pharmacology (2019): 23-29

Glutathione peroxidase and malondialdehyde in children with chronic hepatitis C

Authors:

Khedr, M. A., El-Araby, H. A., Konsowa, H. A., Sokar, S. S., Mahmoud, M. F., Adawy, N. M., Zakaria, H. M.

Journal:

Clin Exp Hepatol (2019): 81-87

Elevated serum levels of malondialdehyde and cortisol are associated with major depressive disorder: A case-control study

Authors:

Islam, M. R., Islam, M. R., Ahmed, I., Moktadir, A. A., Nahar, Z., Islam, M. S., Shahid, S. F. B., Islam, S. N., Islam, M. S., Hasnat, A.

Journal:

SAGE Open Med (2018): 2050312118773953

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	480 nm
Emission	555 nm
Cutoff	530 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: MDA Green™	2 vials
Component B: MDA Assay Buffer	1 bottle (20 mL)
Component C: MDA Standard	1 vial
Component D: MDA Stopping Solution	1 bottle (10 mL)
Component E: DMSO	1 vial (100 uL)