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Amplite® Fluorimetric Mercuric Ion Quantitation Kit
Mercuric ion is an essential metal ion that plays an important role in a number of biological processes. Mercury ion is considered to be one of the most hazardous pollutants and highly dangerous materials. The high toxicity of Hg2+ is caused by its high affinity to the thiol groups in biological ligands such as proteins, DNA, and enzymes. When Hg2+ is absorbed in the human body from the environment, it induces aberrations in microtubules, ion channels, and mitochondria presumably and significant damage to the kidneys, heart, brain, stomach, intestines, central nervous system and immune systems. In addition, mercury accumulates through food chains or atmosphere in the ecological system, and has a relatively long atmospheric residence time because of its non-biodegradation. It has been intensively studies for a variety reasons, in particular, its environmental impacts and biological complications. However, there are very few commercial products that were developed for rapid detection mercuric ion. The highly selective and sensitive detection of mercury is of toxicological and environmental importance. Amplite® Fluorimetric Mercuric Ion Quantitation Kit offers a robust fluorescence-based assay for measuring mercury ion (Hg2+) with high selectivity. Mercury Lite™ 590 itself is nearly non-fluorescent, but generates more than 500-fold fluorescence enhancement upon binding Hg2+ ion. The fluorescence signal can be measured with a fluorescence microplate reader. With this kit, we were able to detect as low as 8 µM Hg2+ in a 100 µL reaction volume.
Hg2+ was measured with the Amplite® Fluorimetric Mercuric Ion Quantitation Kit (Cat#19005) in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices). As low as 8 µM mercury (II) perchlorate was detected with 30 minutes incubation.
Hg2+ was measured with the Amplite® Fluorimetric Mercuric Ion Quantitation Kit (Cat#19005) in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices). As low as 8 µM mercury (II) perchlorate was detected with 30 minutes incubation.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
19005100 tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
UNSPSC41116134
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on September 24, 2025