Name: Amplite® Fluorimetric Mercuric Ion Quantitation Kit
Brand: AAT Bioquest
SKU: 19005
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Mercuric Ion Quantitation Kit

Amplite® Fluorimetric Mercuric Ion Quantitation Kit is a fluorimetric assay kit for quantification of mercury (II) ions in solution.

Detection Principle: Mercury Lite™ 590 becomes strongly fluorescent upon binding Hg²⁺.
Sensitivity: Detects Hg²⁺ down to 8 µM in a 100 µL reaction.
Signal Advantage: Provides >500-fold fluorescence enhancement upon Hg²⁺ binding.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.

Hg2+ was measured with the Amplite® Fluorimetric Mercuric Ion Quantitation Kit (Cat#19005) in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices). As low as 8 µM mercury (II) perchlorate was detected with 30 minutes incubation.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
19005	100 tests	Price

Overview

Mercuric ion is an essential metal ion that plays an important role in a number of biological processes. Mercury ion is considered to be one of the most hazardous pollutants and highly dangerous materials. The high toxicity of Hg2+ is caused by its high affinity to the thiol groups in biological ligands such as proteins, DNA, and enzymes. When Hg2+ is absorbed in the human body from the environment, it induces aberrations in microtubules, ion channels, and mitochondria presumably and significant damage to the kidneys, heart, brain, stomach, intestines, central nervous system and immune systems. In addition, mercury accumulates through food chains or atmosphere in the ecological system, and has a relatively long atmospheric residence time because of its non-biodegradation. It has been intensively studies for a variety reasons, in particular, its environmental impacts and biological complications. However, there are very few commercial products that were developed for rapid detection mercuric ion. The highly selective and sensitive detection of mercury is of toxicological and environmental importance. Amplite® Fluorimetric Mercuric Ion Quantitation Kit offers a robust fluorescence-based assay for measuring mercury ion (Hg2+) with high selectivity. Mercury Lite™ 590 itself is nearly non-fluorescent, but generates more than 500-fold fluorescence enhancement upon binding Hg2+ ion. The fluorescence signal can be measured with a fluorescence microplate reader. With this kit, we were able to detect as low as 8 µM Hg2+ in a 100 µL reaction volume.

References

View all 11 references: Citation Explorer

Coordination of mercury (II) to gold nanoparticle associated nitrotriazole towards sensitive colorimetric detection of mercuric ion with a tunable dynamic range

Authors:

Chen, Xiaojun and Zu, Yanbing and Xie, Hong and Kemas, Aurino Muhammad and Gao, Zhiqiang

Journal:

Analyst (2011): 1690--1696

Direct measurement of mercury (II) removal from organomercurial lyase (MerB) by tryptophan fluorescence: NmerA domain of coevolved γ-proteobacterial mercuric ion reductase (MerA) is more efficient than MerA catalytic core or glutathione

Authors:

Hong, Baoyu and Nauss, Rachel and Harwood, Ian M and Miller, Susan M

Journal:

Biochemistry (2010): 8187--8196

Sequence and analysis of a plasmid-encoded mercury resistance operon from Mycobacterium marinum identifies MerH, a new mercuric ion transporter

Authors:

Schué, Mathieu and Dover, Lynn G and Besra, Gurdyal S and Parkhill, Julian and Brown, Nigel L

Journal:

Journal of bacteriology (2009): 439--444

Is the cytoplasmic loop of MerT, the mercuric ion transport protein, involved in mercury transfer to the mercuric reductase?

Authors:

Rossy, Emmanuel and Sénèque, Olivier and Lascoux, David and Lemaire, David and Crouzy, Serge and Delangle, Pascale and Covès, Jacques

Journal:

FEBS letters (2004): 86--90

MerF is a mercury transport protein: different structures but a common mechanism for mercuric ion transporters?

Authors:

Wilson, Jon R and Leang, Ching and Morby, Andrew P and Hobman, Jon L and Brown, Nigel L

Journal:

FEBS letters (2000): 78--82

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Mercury Lite™ 590	1 vial
Component B: Assay Buffer	1 bottle (10 mL)
Component C: DMSO	1 vial (100 µL)