Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Myeloperoxidase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence* is a fluorimetric assay kit for measurement of myeloperoxidase activity in solution and cell lysates.

Detection Principle: Measures myeloperoxidase activity using an Amplite® Red-based fluorescent reaction.
Sensitivity: Detects myeloperoxidase activity down to 0.1 mU/mL in a 100 µL reaction.
Workflow: Compatible with 96- or 384-well microplates and automation without a separation step.
Instrument Compatibility: Supports fluorescence detection at Ex/Em 540/590 nm and can be easily adapted to automation.

Myeloperoxidase dose response was measured with Amplite® Fluorimetric Myeloperoxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11301	200 Tests	Price

Overview

Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity. It catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates; and plays an important role in the oxygen-dependent killing of tumor cells and microorganisms. MPO deficiency is a hereditary deficiency of the enzyme, which predisposes to immune deficiency. There are considerable interests in the development of therapeutic MPO inhibitors. Our Amplite® Myeloperoxidase Assay Kit provides a quick and sensitive method for the measurement of myeloperoxidase in solution and in cell lysates. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Myeloperoxidase Assay Kit, we have detected as little as 0.1 mU/ml myeloperoxidase in a 100 µL reaction volume. The kit can be automated for high throughput screenings of MPO inhibitors.

Citations

View all 2 citations: Citation Explorer

Fullerene and fullerene whisker are not carcinogenic to the lungs and pleura in rat long-term study after 2-week intra-tracheal intrapulmonary administration

Authors:

Sheema, Asraful Nahar and Naiki-Ito, Aya and Kakehashi, Anna and Ahmed, Omnia Hosny Mohamed and Alexander, David B and Alexander, William T and Numano, Takamasa and Kato, Hiroyuki and Goto, Yuko and Takase, Hiroshi and others,

Journal:

Archives of Toxicology (2024): 1--16

Influence of Dinitrosyl Iron Complexes on the Activity of Enzymes, Indicators of Cardiovascular Diseases

Authors:

Akentieva, Natalia and Sanina, Natalia and Gizatullin, Artur and Shkondina, Natalia and Andreeva, Anna and Shram, Stanislav and Aldoshin, Sergei

References

View all 27 references: Citation Explorer

Detection of myeloperoxidase activity in primary leukemic cells by an enhanced chemiluminescent assay for differentiation between acute lymphoblastic and non-lymphoblastic leukemia

Authors:

Tan S, Wang G, Peng M, Zhang X, Shen G, Jiang J, Chen F.

Journal:

Clin Chim Acta (2009): 216

Determination of myeloperoxidase in EDTA plasma: comparison of an enzyme-linked immunosorbent assay with a chemiluminescent automated immunoassay

Authors:

Zelzer S, Khoschsorur G, Stettin M, Weihrauch G, Truschnig-Wilders M.

Journal:

Clin Chim Acta (2009): 62

Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease

Authors:

Grulke S, Franck T, Gangl M, Peters F, Salciccia A, Deby-Dupont G, Serteyn D.

Journal:

Can J Vet Res (2008): 37

Measurement of equine myeloperoxidase (MPO) activity in synovial fluid by a modified MPO assay and evaluation of joint diseases - an initial case study

Authors:

Fietz S, Bondzio A, Moschos A, Hertsch B, Einspanier R.

Journal:

Res Vet Sci (2008): 347

A novel assay system for myeloperoxidase activity in whole saliva

Authors:

Sakamoto W, Fujii Y, Kanehira T, Asano K, Izumi H.

Journal:

Clin Biochem (2008): 584

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: H2O2	1 vial (100 µL, 3%)
Component D: Myeloperoxidase Standard	1 vial (10 mU, lyophilized)
Component E: DMSO	1 vial (200 µL)