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Amplite® Fluorimetric NADH Assay Kit
Red Fluorescence
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite® NADH Assay Kit provides a convenient method for sensitive detection of NADH. The enzymes in the system specifically recognize NADH in an enzyme cycling reaction. The enzyme cycling reaction significantly increases detection sensitivity.
NADH dose response was measured with Amplite® Fluorimetric NADH Assay Kit in a 96-well solid black plate using a NOVOStar microplate reader (BMG Labtech). RFU measured over Ex/Em = 540/590 nm.
NADH dose response was measured with Amplite® Fluorimetric NADH Assay Kit in a 96-well solid black plate using a NOVOStar microplate reader (BMG Labtech). RFU measured over Ex/Em = 540/590 nm.
CatalogSize
Price
Quantity
15261400 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on September 25, 2025