Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric NADP Assay Kit

Blue Fluorescence

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence* is a fluorimetric assay kit for quantification of NADP in a microplate format.

Detection Principle: Directly measures NADP using the Quest Fluor™ NADP Probe.
Sensitivity: Detects NADP down to 30 nM in a 100 µL assay.
Specificity: The probe reacts selectively with NADP and shows little response to NADPH.
Workflow: Compatible with 96- or 384-well microplates and high-throughput screening.
Instrument Compatibility: Fluorescence detection at Ex/Em 420/480 nm.

NADP standard curve with 100 µM NADPH in presence in the solution. As low as 0.3% of NADP (~300 nM) converted from NADPH can be detected with 20 min incubation (n=3). RFU read at Ex/Em = 420/480 nm.

Protocol

SDS

Catalog	Size	Price	Quantity
15281	200 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which requires NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. Quantifying the generation or consumption of these factors is an important method to monitor the enzyme-mediated reaction or screening the modulator or substrate of these enzyme reactions. There are several kits on the market to quantify NADPH or total NADP/NADPH amount, but detection NADP generation in the presence of large excess amount of NADPH has been quite challenging to date because NADP has its absorption peak at 260 nm and does not fluorescence, making the measurement unpractical. Amplite® Fluorimetric NADP Assay Kit provides a sensitive and rapid detection of NADP. The kit directly measure NADP using Quest Fluor™ NADP reagent, our newly developed NADP sensor. The proprietary probe used in this kit reacts only with NADP to generate a product that fluorescence at a specific excitation and emission spectra range and has little response to NADPH. This kit can detect as little as 30 nM NADP in a 100 µL assay volume, and monitor 0.3% NADP generation in the presence of excess amount of NADPH. This assay can be performed in a convenient 96-well or 384-well microtiter-plate format and can be used in high-throughput screening.

Citations

View all 57 citations: Citation Explorer

Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio

Authors:

Luo, Gang and Huang, Bingqing and Qiu, Xiang and Xiao, Lin and Wang, Ning and Gao, Qin and Yang, Wei and Hao, Liping

Journal:

Molecular Nutrition & Food Research (2017)

Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase

Authors:

Ling, Min and Huang, Peixin and Islam, Shamima and Heruth, Daniel P and Li, Xuanan and Zhang, Li Qin and Li, Ding-You and Hu, Zhaohui and Ye, Shui Qing

Journal:

Cell & Bioscience (2017): 27

MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells

Authors:

Ren, T and Zhang, H and Wang, J and Zhu, J and Jin, M and Wu, Y and Guo, X and Ji, L and Huang, Q and Yang, H and others, undefined

Journal:

Oncogene (2017)

Metabolic and molecular insights into an essential role of nicotinamide phosphoribosyltransferase

Authors:

Zhang, Li Q and Van Ha, undefined and el, Leon and Xiong, Min and Huang, Peixin and Heruth, Daniel P and Bi, Charlie and Gaedigk, Roger and Jiang, Xun and Li, Ding-You and Wyckoff, Gerald and others, undefined

Journal:

Cell Death & Disease (2017): e2705

Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation

Authors:

Yang, Fei and Heit, Caitlin and Inglis, Debra L

Journal:

Fermentation (2017): 61

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	420 nm
Emission	480 nm
Cutoff	455 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Quest Fluor™ NADP Probe	1 bottle (5 mL)
Component B: Assay Solution	1 bottle (5 mL)
Component C: Enhancer Solution	1 bottle (3.5 mL)
Component D: NADP Standard	1 vial (389 µg)