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Amplite® Fluorimetric NADP Assay Kit
Blue Fluorescence
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which requires NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. Quantifying the generation or consumption of these factors is an important method to monitor the enzyme-mediated reaction or screening the modulator or substrate of these enzyme reactions. There are several kits on the market to quantify NADPH or total NADP/NADPH amount, but detection NADP generation in the presence of large excess amount of NADPH has been quite challenging to date because NADP has its absorption peak at 260 nm and does not fluorescence, making the measurement unpractical. Amplite® Fluorimetric NADP Assay Kit provides a sensitive and rapid detection of NADP. The kit directly measure NADP using Quest Fluor™ NADP reagent, our newly developed NADP sensor. The proprietary probe used in this kit reacts only with NADP to generate a product that fluorescence at a specific excitation and emission spectra range and has little response to NADPH. This kit can detect as little as 30 nM NADP in a 100 µL assay volume, and monitor 0.3% NADP generation in the presence of excess amount of NADPH. This assay can be performed in a convenient 96-well or 384-well microtiter-plate format and can be used in high-throughput screening.
NADP standard curve with 100 µM NADPH in presence in the solution. As low as 0.3% of NADP (~300 nM) converted from NADPH can be detected with 20 min incubation (n=3). RFU read at Ex/Em = 420/480 nm.
NADP standard curve with 100 µM NADPH in presence in the solution. As low as 0.3% of NADP (~300 nM) converted from NADPH can be detected with 20 min incubation (n=3). RFU read at Ex/Em = 420/480 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
15281200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation420 nm
Emission480 nm
Cutoff455 nm
Recommended plateSolid black
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Page updated on October 13, 2025