logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit
The Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity. This assay is based on the proteolytic cleavage of a synthetic substrate by NE, which can be quantified at Ex/Em= 360/470 nm. It offers a direct measure of enzymatic activity proportional to the NE present in the samples. The kit’s straightforward protocol is optimized for the sensitive detection of NE in blood and plasma samples and is suitable for screening and characterizing chemotactic agents triggering neutrophil stimulation. Neutrophil elastase, also known as leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil, elaszym, serine elastase, subtype human leukocyte elastase (HLE) is a cytotoxic Serine Protease with a broad substrate specificity. Azurophil granules of neutrophil store NE and release them in response to multiple stimuli like pathogens, immune complexes, or chemotactic agents such as PMA causing degradation of a range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. NE hydrolysis accounts for approximately 80% of the total protease hydrolysis activity in the human body. Disease implications of NE include cystic fibrosis, COPD, lung emphysema, rheumatoid arthritis, and adult respiratory distress syndrome. NE has also been associated with lung injury seen in COVID-19.
The dose response of neutrophil elastase (NE) was measured using the Amplite® Fluorimetric Neutrophil Elastase Assay Kit. The assay was conducted on a 96-well solid black microplate, utilizing a Gemini microplate reader (Molecular Devices) with Ex/Em = 360/470 nm, Cutoff = 430 nm.
The dose response of neutrophil elastase (NE) was measured using the Amplite® Fluorimetric Neutrophil Elastase Assay Kit. The assay was conducted on a 96-well solid black microplate, utilizing a Gemini microplate reader (Molecular Devices) with Ex/Em = 360/470 nm, Cutoff = 430 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11327100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation360 nm
Emission470 nm
Cutoff430 nm
Recommended plateSolid black
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on December 11, 2025