Name: Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit
Brand: AAT Bioquest
SKU: 11327
Price: 412 USD
Availability: InStock

Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit

Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit is a fluorimetric assay kit for quantification of neutrophil elastase activity in blood and plasma samples.

Detection Principle: Measures NE-mediated proteolytic cleavage of a synthetic fluorescent substrate.
Workflow: Incubates for 10–20 minutes at 37°C.
Instrument Compatibility: Fluorescence detection at Ex/Em 360/470 nm with a 430 nm cutoff with a microplate reader.
Applications: Suitable for screening and characterizing chemotactic agents that trigger neutrophil stimulation.

The dose response of neutrophil elastase (NE) was measured using the Amplite® Fluorimetric Neutrophil Elastase Assay Kit. The assay was conducted on a 96-well solid black microplate, utilizing a Gemini microplate reader (Molecular Devices) with Ex/Em = 360/470 nm, Cutoff = 430 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11327	100 Tests	Price

Overview

The Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity. This assay is based on the proteolytic cleavage of a synthetic substrate by NE, which can be quantified at Ex/Em= 360/470 nm. It offers a direct measure of enzymatic activity proportional to the NE present in the samples. The kit’s straightforward protocol is optimized for the sensitive detection of NE in blood and plasma samples and is suitable for screening and characterizing chemotactic agents triggering neutrophil stimulation. Neutrophil elastase, also known as leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil, elaszym, serine elastase, subtype human leukocyte elastase (HLE) is a cytotoxic Serine Protease with a broad substrate specificity. Azurophil granules of neutrophil store NE and release them in response to multiple stimuli like pathogens, immune complexes, or chemotactic agents such as PMA causing degradation of a range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. NE hydrolysis accounts for approximately 80% of the total protease hydrolysis activity in the human body. Disease implications of NE include cystic fibrosis, COPD, lung emphysema, rheumatoid arthritis, and adult respiratory distress syndrome. NE has also been associated with lung injury seen in COVID-19.

References

View all 49 references: Citation Explorer

Investigating Polypharmacology through Targeting Known Human Neutrophil Elastase Inhibitors to Proteinase 3.

Authors:

Gartan, Parveen and Khorsand, Fahimeh and Mizar, Pushpak and Vahokovski, Juha Ilmari and Cervantes, Luis F and Haug, Bengt Erik and Brenk, Ruth and Brooks, Charles L and Reuter, Nathalie

Journal:

Journal of chemical information and modeling (2024): 621-626

Human neutrophil elastase inhibitors: Classification, biological-synthetic sources and their relevance in related diseases.

Authors:

Ocampo-Gallego, Jhonnatan Styver and Pedroza-Escobar, David and Caicedo-Ortega, Ana Ruth and Berumen-Murra, María Teresa and Novelo-Aguirre, Ana Lucía and de Sotelo-León, Rebeca Denis and Delgadillo-Guzmán, Dealmy

Journal:

Fundamental & clinical pharmacology (2024): 13-32

Elevated first-trimester neutrophil elastase and proteinase 3 increase the risk of gestational diabetes mellitus and adverse fetal outcomes.

Authors:

Wang, Lihong and Zhou, Zhoujunhao and Xu, Xinming and Li, Yue and Zhang, Rui and Yu, Zhiyan and Huang, Xinmei and Zang, Shufei and Sun, Tiange

Journal:

Reproductive biology and endocrinology : RB&E (2024): 2

Neutrophil Elastase Degrades Histone Deacetylases and Sirtuin 1 in Primary Human Monocyte Derived Macrophages.

Authors:

Zheng, Shuo and Bulut, Gamze B and Kummarapurugu, Apparao B and Ma, Jonathan and Voynow, Judith A

Journal:

International journal of molecular sciences (2024)

Botulinum toxin type A ameliorates rat dorsal root ganglia neuron pyroptosis in postherpetic neuralgia by upregulating cathelicidin antimicrobial peptide to inhibit neutrophil elastase.

Authors:

Wan, Quan

Journal:

Chemical biology & drug design (2024): e14406

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	360 nm
Emission	470 nm
Cutoff	430 nm
Recommended plate	Solid black

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Page updated on October 26, 2026

Component A: NE Substrate	50 µL
Component B: NE Enzyme Standard	1 µg
Component C: NE Dilution Buffer	5 mL
Component D: NE Assay Buffer	5 mL