Name: Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 13841
Price: 415 USD
Availability: InStock

Amplite® Fluorimetric Oxaloacetate Assay Kit

Red Fluorescence

Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of oxaloacetate in biological samples.

Detection Principle: Converts oxaloacetate to pyruvate, generating H₂O₂ through an enzyme-coupled reaction.
Detection Chemistry: Uses Amplite® Red to measure the generated H₂O₂.
Workflow: Incubates at room temperature for 30–60 minutes.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.

Oxaloacetate dose response was measured with the Amplite® Fluorimetric Oxaloacetate Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13841	200 Tests	Price

Overview

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate. It is also involved in gluconeogenesis, urea cycle, glyoxylate cycle, amino acid synthesis, and fatty acid synthesis. The lack of oxaloacetate limits gluconeogenesis and urea cycle function, and can lead to decreased production of energy. Oxaloacetate can be also used as blood glutamate scavengers to provide neuroprotection after traumatic brain injury, expressed both by reduced neuronal loss in the hippocampus and improved neurologic outcomes. Amplite® Fluorimetric Oxaloacetate Assay Kit offers a sensitive assay for quantifying oxaloacetate in biological samples. Oxaloacetate is converted to pyruvate that generates hydrogen peroxide through an enzyme coupled reaction. The production of hydrogen peroxide is monitored with Amplite® Red by a fluorescence microplate reader.

References

View all 17 references: Citation Explorer

Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase

Authors:

Kato S, Ikuta T, Hemmi H, Takahashi S, Kera Y, Yoshimura T.

Journal:

Biosci Biotechnol Biochem (2012): 2150

Assay of blood and tissue oxaloacetate and alpha-ketoglutarate by isotope dilution gas chromatography-mass spectrometry

Authors:

Laplante A, Comte B, Des Rosiers C.

Journal:

Anal Biochem (1995): 580

Effect of oxaloacetate and phosphorylation on ATP-citrate lyase activity

Authors:

Pentyala SN, Benjamin WB.

Journal:

Biochemistry (1995): 10961

Novel oxaloacetate effect on mitochondrial Ca2+ movement

Authors:

Leikin YN, Zharova TV, Tjulina OV.

Journal:

FEBS Lett (1993): 35

A sensitive multienzymatic assay for the measurement of pyruvate, dihydroxyacetone phosphate, oxaloacetate, and acetoacetate in clear extracts from biological samples

Authors:

Arias-Mendoza F, Pina E.

Journal:

Prep Biochem (1991): 211

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (20 mL)
Component D: Oxaloacetate Decarboxcylase (OAC)	2 vials (lyophilized powder)
Component E: Oxaloacetate Standard	1 vial (lyophilized powder)
Component F: DMSO	1 vial (100 µL)