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Amplite® Fluorimetric Protein Quantitation Kit
Orange Fluorescence
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications. Biuret, Lowry, BCA and Bradford assays are routinely used for estimating protein concentration. However, these colorimetric assays are less sensitive, and require large sample volume to ensure accuracy. Our Amplite® Fluorimetric Protein Quantitation Kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays. Prolite™ Orange used in the kit is non-fluorescent in aqueous solution, but reacts rapidly with proteins and generates bright fluorescence. The Amplite® Fluorimetric Protein Quantitation Kit provides a simple method for quantifying protein concentration in solutions. As little as 0.1 µg/mL of BSA can be detected. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format. It can be completed within 30 minutes with the fluorescence signal easily monitored. This kit has been used for (1) studying protein/protein interactions; (2) measuring column fractions after affinity chromatography; (3) estimating recovery of membrane proteins from cell extract; and (4) high-throughput screening of fusion proteins.
<p>BSA, chicken-egg ovalbumin, porcine thyroglobulin dose response was measured at Ex/Em 485/590 in a 96-well black plate with the Amplite® Fluoremetric Protein Quantitation Kit. As low as 0.1 ug/mL of BSA can be detected.</p>
<p>BSA, chicken-egg ovalbumin, porcine thyroglobulin dose response was measured at Ex/Em 485/590 in a 96-well black plate with the Amplite® Fluoremetric Protein Quantitation Kit. As low as 0.1 ug/mL of BSA can be detected.</p>
CatalogSize
Price
Quantity
11105500 tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation485 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 13, 2025