Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Amplite® Fluorimetric Protein Quantitation Kit

Orange Fluorescence

Amplite® Fluorimetric Protein Quantitation Kit is a fluorimetric assay kit for quantification of protein concentration in solution.

Detection Principle: Prolite™ Orange reacts with proteins to generate bright orange fluorescence.
Sensitivity: Detects BSA down to 0.1 µg/mL.
Signal Advantage: Provides greater sensitivity than conventional Bradford and BCA colorimetric assays.
Instrument Compatibility: Fluorescence detection at Ex/Em 485/590 nm using solid black microplates.

<p>BSA, chicken-egg ovalbumin, porcine thyroglobulin dose response was measured at Ex/Em 485/590 in a 96-well black plate with the Amplite® Fluoremetric Protein Quantitation Kit. As low as 0.1 ug/mL of BSA can be detected.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11105	500 tests	Price

Overview

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications. Biuret, Lowry, BCA and Bradford assays are routinely used for estimating protein concentration. However, these colorimetric assays are less sensitive, and require large sample volume to ensure accuracy. Our Amplite® Fluorimetric Protein Quantitation Kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays. Prolite™ Orange used in the kit is non-fluorescent in aqueous solution, but reacts rapidly with proteins and generates bright fluorescence. The Amplite® Fluorimetric Protein Quantitation Kit provides a simple method for quantifying protein concentration in solutions. As little as 0.1 µg/mL of BSA can be detected. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format. It can be completed within 30 minutes with the fluorescence signal easily monitored. This kit has been used for (1) studying protein/protein interactions; (2) measuring column fractions after affinity chromatography; (3) estimating recovery of membrane proteins from cell extract; and (4) high-throughput screening of fusion proteins.

References

View all 27 references: Citation Explorer

Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination

Authors:

Samokhvalov, A. V.; Safenkova, I. V.; Eremin, S. A.; Zherdev, A. V.; Dzantiev, B. B.

Journal:

Anal Chim Acta (2017): 80-87

Quantification of Membrane Protein Self-Association with a High-Throughput Compatible Fluorescence Assay

Authors:

Li, J.; Qiu, X. J.

Journal:

Biochemistry (2017): 1951-1954

Dual Amplification Fluorescence Assay for Alpha Fetal Protein Utilizing Immunohybridization Chain Reaction and Metal-Enhanced Fluorescence of Carbon Nanodots

Authors:

Xu, D. D.; Liu, C.; Li, C. Y.; Song, C. Y.; Kang, Y. F.; Qi, C. B.; Lin, Y.; Pang, D. W.; Tang, H. W.

Journal:

ACS Appl Mater Interfaces (2017): 37606-37614

Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria

Authors:

Akbar, S. M.; Sreeramulu, K.; Sharma, H. C.

Journal:

J Bioenerg Biomembr (2016): 241-7

Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe

Authors:

Song, W.; Wang, Y.; Liang, R. P.; Zhang, L.; Qiu, J. D.

Journal:

Biosens Bioelectron (2015): 234-40

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	485 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 27, 2026

Component A: 500X Prolite™ Orange	1 vial (50 µL)
Component B: BSA Standard (1 mg/mL)	1 vial (0.5 mL)
Component C: Protein Enhancer	1 vial
Component D: Assay Buffer	1 bottle (50 mL)
Component E: Protein Enhancer Buffer	1 vial (100 µL)