Name: Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 13530
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Renin Assay Kit

Red Fluorescence

Amplite® Fluorimetric Renin Assay Kit is a fluorimetric assay kit for detection of renin activity in biological samples and inhibitor screening assays.

Detection Principle: Renin cleavage of a FRET peptide restores red fluorescence.
Sensitivity: Detects as little as 1 ng renin in a 100 µL reaction.
Detection Chemistry: Uses a Tide Fluor™ 3/Tide Quencher™ 3 FRET peptide substrate.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.
Applications: Supports renin activity measurement and high-throughput screening of renin inhibitors.

Renin dose response was measured with Amplite® Fluorimetric Renin Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13530	100 tests	Price

Overview

Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction. It regulates blood pressure and electrolyte homoeostasis. Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. Renin has been identified to be an attractive target for the treatment of hypertension. The Amplite® Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and renin activity using our proprietary Tide Fluor™ 3 (TF3)/Tide Quencher™ 3 (TQ3) fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, and the fluorescent signal can be easily monitored by a fluorescence microplate reader. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay. With the Amplite® Renin Assay Kit, we have detected as little as 1ng renin in a 100 µL reaction volume. However, the selectivity of the rennin substrate used in the kit has not been thoroughly tested. It may also response to other proteases since peptide-based protease substrates generally have low selectivity.

References

View all 70 references: Citation Explorer

Activation of the succinate receptor GPR91 in macula densa cells causes renin release

Authors:

Vargas SL, Toma I, Kang JJ, Meer EJ, Peti-Peterdi J.

Journal:

J Am Soc Nephrol (2009): 1002

Hypotonicity-induced Renin exocytosis from juxtaglomerular cells requires aquaporin-1 and cyclooxygenase-2

Authors:

Friis UG, Madsen K, Svenningsen P, Hansen PB, Gulaveerasingam A, Jorgensen F, Aalkjaer C, Skott O, Jensen BL.

Journal:

J Am Soc Nephrol (2009): 2154

Cytosolic renin is targeted to mitochondria and induces apoptosis in H9c2 rat cardiomyoblasts

Authors:

Wanka H, Kessler N, Ellmer J, Endlich N, Peters BS, Clausmeyer S, Peters J.

Journal:

J Cell Mol Med (2009): 2926

Measurement of plasma renin concentration in cats by use of a fluorescence resonance energy transfer peptide substrate of renin

Authors:

Schmiedt CW, Hurley KA, Tong X, Rakhmanova VA, Po CL, Hurley DJ.

Journal:

Am J Vet Res (2009): 1315

Prolonged exposure of cardiac cells to renin plus angiotensinogen reduces intracellular renin in the failing heart. On the role of angiotensin II-AT1 complex internalization

Authors:

De Mello WC, Gerena Y.

Journal:

Regul Pept (2009): 139

Spectral properties

Excitation (nm)	545
Emission (nm)	572

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 23, 2026

Component A: Renin Red™ Substrate (100X)	1 vial (50 µL)
Component B: Renin Standard	1 vial (40 µg/mL, 25 µL)
Component C: Assay Buffer	1 bottle (10 mL)