Amplite® Fluorimetric Sodium Ion Quantification Kit
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 491 |
Emission (nm) | 511 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Excitation (nm) 491 | Emission (nm) 511 |
Platform
Fluorescence microplate reader
Excitation | 490 nm |
Emission | 525 nm |
Cutoff | 515 nm |
Recommended plate | Solid black |
Components
Example protocol
AT A GLANCE
Add 50 µL NaCl Standards or test samples
Add 50 µL SoNa™ 520 working solution.
Incubate at RT for 5-10 minutes
Monitor the fluorescence at Ex/Em=490/525 nm
The following protocol is an example for quantifying sodium content using SoNa™ 520. Allow all the components to warm to room temperature before opening. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 μL of DMSO (Component D) into SoNa™ 520 vial (Component A).
Note: Make a single unused SoNa™ 520 stock solution aliquot and store at ≤ -20 º C. Protect from light and avoid repeated freeze-thaw cycles.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/21325
PREPARATION OF WORKING SOLUTION
Add 100 μL of SoNa™ 520 (Component A) into 5 mL of Assay Buffer (Component B). Protect the working solution from light by covering it with foil or placing it in the dark.
Note: For best results, this solution should be used within a few hours of its preparation.
Note: 5 mL of working solution is enough for 100 tests.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol only provides a guideline and should be modified according to your specific needs.
SS=NaCl Standards (SS1 - SS7, 40 to 0.625 mM, 2X dilutions); BL=Blank Control; TS=Test Samples
BL | BL | TS | TS |
SS1 | SS1 | ... | ... |
SS2 | SS2 | ... | ... |
SS3 | SS3 | ... | ... |
SS4 | SS4 | ... | ... |
SS5 | SS5 | ... | ... |
SS6 | SS6 | ... | ... |
SS7 | SS7 | ... | ... |
Well | Volume | Reagent |
SS1-SS7 | 50 µL | Serial dilutions (40 to 0.625 mM) |
BL | 50 µL | Assay Buffer |
TS | 50 µL | Sample |
Prepare NaCl standards (SS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384-well plate, use 25 µL of reagent per well instead of 50 µL.
Add 50 µL of SoNa™ 520 working solution to each well of NaCl standards, blank control, and test samples to make the assay volume of 100 µL/well. For a 384-well plate, add 25 µL into each well instead, for a total volume of 50 µL/well.
Incubate the reaction at room temperature for 5 to 10 minutes, protected from light.
Monitor the fluorescence increase with a fluorescence microplate reader at Ex/Em = 490/525 nm (cut off at 515 nm).
Product Family
Name | Excitation (nm) | Emission (nm) |
Amplite® Fluorimetric Lithium Ion Quantification Kit | 491 | 513 |
Portelite™ Fluorimetric Sodium Ion Quantification Kit | 491 | 511 |
Images
References
Authors: Kushi, Efrem Negash and Belachew, Tefera and Tamiru, Dessalegn
Journal: Journal of nutritional science (2023): e5
Authors: Jiménez Rodríguez, Amelia and Palomo Cobos, Luis and Rodríguez-Martín, Amelia and Fernández Del Valle, Patricia and Novalbos-Ruíz, José P
Journal: Nutrients (2023)
Authors: Vera, Michael A and Sutphin, Angela and Hansen, Lisa and El-Khoury, Joe M
Journal: Laboratory medicine (2022): e105-e108
Authors: Evenepoel, A and Herroelen, P and Lanckmans, K and van der Werff Ten Bosch, J and Martin, M and Weets, I and van Dalem, A
Journal: Acta clinica Belgica (2022): 832-836
Authors: Ouatu, Anca and Mihai, Madalina Stefana and Tanase, Daniela Maria and Dascalu, Cristina Gena and Dima, Nicoleta and Serban, Lacramioara Ionela and Rezus, Ciprian and Floria, Mariana
Journal: Diagnostics (Basel, Switzerland) (2021)
Authors: Jia, Mengtian and Zhu, Yanyan and Guo, Dong and Bi, Xiaogang and Hou, Xiaohong
Journal: Analytica chimica acta (2020): 211-220
Authors: Tischer, Bruna and Teixeira, Iberê Damê and Filoda, Paula Freitas and Alessio, Keiti Oliveira and Barin, Juliano Smanioto and Duarte, Fábio Andrei and Kipper, Liane Mahlmann and Helfer, Gilson Augusto and Costa, Adilson Ben da
Journal: Food chemistry (2020): 125456
Authors: Barkhuizen, Marizna and Hoffmann, Mariza and Zöllner, Ekkehard Wa and Erasmus, Rajiv T and Zemlin, Annalise E
Journal: Biochemia medica (2019): 011002
Authors: Timmons, Andrew K and Korpak, Anna M and Tan, Jenny and Moore, Kathryn P and Liu, Cindy H and Forsberg, Christopher W and Goldberg, Jack and Smith, Nicholas L and Cohen, David M
Journal: American journal of physiology. Renal physiology (2019): F1114-F1123
Authors: Gazulla, María Fernanda and Andreu, Cristina and Rodrigo, Marta and Orduña, Mónica and Ventura, María Jesús
Journal: Talanta (2018): 600-605
Application notes
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