Name: Amplite® Fluorimetric Sodium Ion Quantification Kit
Brand: AAT Bioquest
SKU: 21325
Price: 527 USD
Availability: InStock

Amplite® Fluorimetric Sodium Ion Quantification Kit

Amplite® Fluorimetric Sodium Ion Quantification Kit is a fluorimetric assay kit for quantification of sodium ions in a microplate format using small sample volumes.

Detection Principle: SoNa™ 520 exhibits increased fluorescence upon binding sodium ions.
Signal Advantage: Provides strong fluorescence enhancement with high selectivity for sodium ions.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm using solid black microplates.

Sodium dose response was measured with Amplite® Fluorimetric Sodium Ion Kit in a 96-well solid black plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21325	100 Tests	Price

Overview

Quantifying sodium ions is important in various scientific fields and industries, including biochemistry, medicine, environmental analysis, and food science etc. There are several methods commonly used to quantify sodium ions, including flame photometry, ion-selective electrodes (ISE), atomic absorption spectroscopy and fluorescence spectrophotometry. Flame photometry and atomic absorption spectroscopy require the inflammation of the samples. They are tedious to use and require expensive and sophisticated instrumentation. Ion-selective electrodes require large volumes of samples and often have low selectivity. Among all the methods, fluorescence spectrophotometry is the most convenient method for quantifying sodium ions. Fluorescence spectrophotometry involves complexing sodium ions with specific reagents and measuring the resulting fluorescence changes. Amplite® Fluorimetric Sodium Ion Quantification Kit uses our robust sodium ion indicator dye, SoNa™ 520, which exhibit great fluorescence intensity enhancement upon binding to sodium ions. SoNa™ 520 is perhaps the most robust sodium ion indicator with high selectivity. It enables the kit to be useful for the rapid determination of sodium concentrations in a variety of samples compared to the other commercial sodium ion assays. This microplate-based assay kit requires extremely small amount of sample, it is particularly suitable for the determination of sodium ion concentration in microvolume format.

References

View all 50 references: Citation Explorer

Importance of palm's heart for pregnant women.

Authors:

Kushi, Efrem Negash and Belachew, Tefera and Tamiru, Dessalegn

Journal:

Journal of nutritional science (2023): e5

Design of a Nutritional Survey to Detect High Dietary Salt Intakes and Its Usefulness in Primary Care Compared to 24-Hour Urine Sodium Determination.

Authors:

Jiménez Rodríguez, Amelia and Palomo Cobos, Luis and Rodríguez-Martín, Amelia and Fernández Del Valle, Patricia and Novalbos-Ruíz, José P

Journal:

Nutrients (2023)

Resolving Pseudohyponatremia: Validation of Plasma Sodium on Radiometer ABL800 Blood Gas Analyzers for Immediate Reflex Testing.

Authors:

Vera, Michael A and Sutphin, Angela and Hansen, Lisa and El-Khoury, Joe M

Journal:

Laboratory medicine (2022): e105-e108

Asparaginase-induced pseudohyponatremia, a case-driven working strategy in pediatric patients.

Authors:

Evenepoel, A and Herroelen, P and Lanckmans, K and van der Werff Ten Bosch, J and Martin, M and Weets, I and van Dalem, A

Journal:

Acta clinica Belgica (2022): 832-836

Hyponatremia-Long-Term Prognostic Factor for Nonfatal Pulmonary Embolism.

Authors:

Ouatu, Anca and Mihai, Madalina Stefana and Tanase, Daniela Maria and Dascalu, Cristina Gena and Dima, Nicoleta and Serban, Lacramioara Ionela and Rezus, Ciprian and Floria, Mariana

Journal:

Diagnostics (Basel, Switzerland) (2021)

Spectral properties

Excitation (nm)	491
Emission (nm)	511

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 12, 2026

Component A: SoNa™ 520	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: NaCl Standard	1 vial
Component D: DMSO	1 vial (100 µL)