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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit
Green Fluorescence
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. The monitoring of reduced (GSH) and oxidized glutathione in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Disorders of cysteine metabolism include cystinosis, an autosomal recessive disease produced by a defect in lysosomal transport, and cystinuria, a common heritable disorder of amino acid transport. There are few reagents or assay kits available for quantitating thiols in biological systems. However, all the commercial kits either lack sensitivity or have tedious protocols. Our Amplite® Fluorimetric Total Thiol Qutitation Kit provides an ultrasensitive fluorimetric assay for quantitating thiols that exist either in a small molecule. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with thiol. The kit provides a sensitive, one-step fluorimetric method to detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM in concentration). The assay is rapid and robust. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. For rapid quantifying thiol groups in a protein, we recommend you use our kit #5529 that is optimized for quantifying protein thiols.
GSH and cysteine dose responses were measured on a solid black 96-well plate with Amplite® Fluorimetric Thiol Quantitation Assay Kit using a NOVOstar microplate reader (BMG Labtech).
GSH and cysteine dose responses were measured on a solid black 96-well plate with Amplite® Fluorimetric Thiol Quantitation Assay Kit using a NOVOstar microplate reader (BMG Labtech).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
5524200 Tests
Price
 
Spectral properties
Excitation (nm)505
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on October 4, 2025