Name: Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 5524
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Total Thiol Quantitation Assay Kit

Green Fluorescence

Detection Principle: Thiolite™ Green becomes strongly fluorescent upon reaction with thiols.
Sensitivity: Detects as little as 1 pmol of cysteine or GSH (10 nM) in a 100 µL assay.
Workflow: One-step assay compatible with 96- or 384-well microplates and can be adapted to automation.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm using solid black microplates.

GSH and cysteine dose responses were measured on a solid black 96-well plate with Amplite® Fluorimetric Thiol Quantitation Assay Kit using a NOVOstar microplate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
5524	200 Tests	Price

Overview

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. The monitoring of reduced (GSH) and oxidized glutathione in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Disorders of cysteine metabolism include cystinosis, an autosomal recessive disease produced by a defect in lysosomal transport, and cystinuria, a common heritable disorder of amino acid transport. There are few reagents or assay kits available for quantitating thiols in biological systems. However, all the commercial kits either lack sensitivity or have tedious protocols. Our Amplite® Fluorimetric Total Thiol Qutitation Kit provides an ultrasensitive fluorimetric assay for quantitating thiols that exist either in a small molecule. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with thiol. The kit provides a sensitive, one-step fluorimetric method to detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM in concentration). The assay is rapid and robust. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. For rapid quantifying thiol groups in a protein, we recommend you use our kit #5529 that is optimized for quantifying protein thiols.

Citations

View all 5 citations: Citation Explorer

Synergistic effects of hydrophilic residues in the transmembrane region on lipid scrambling activity of dimeric helices

Authors:

Nakao, Hiroyuki and Tsujii, Toshiki and Saito, Hiroaki and Ikeda, Keisuke and Nakano, Minoru

Journal:

Colloids and Surfaces B: Biointerfaces (2025): 114612

Perlecan-targeted nanoparticles for drug delivery to triple-negative breast cancer

Authors:

Khanna, Vidhi and Kalscheuer, Stephen and Kirtane, Ameya and Zhang, Wenqui and Panyam, Jayanth

Journal:

Future Drug Discovery (2019)

Improving Payload Capacity and Anti-Tumor Efficacy of Mesenchymal Stem Cells Using TAT Peptide Functionalized Polymeric Nanoparticles

Authors:

Moku, Gopikrishna and Layek, Buddhadev and Trautman, Lana and Putnam, Samuel and Panyam, Jayanth and Prabha, Swayam

Journal:

Cancers (2019): 491

Antibody Conjugated Nanoparticles for Targeting Metastatic Triple Negative Breast Cancer

Authors:

Khanna, Vidhi Devendra

Activation of transcription factors in human bronchial epithelial cells exposed to aqueous extracts of mainstream cigarette smoke in vitro

Authors:

Sekine, Takashi and Hirata, Tadashi and Mine, Toshiki and Fukano, Yasuo

Journal:

Toxicology mechanisms and methods (2016): 22--31

References

View all 26 references: Citation Explorer

Covalent surface chemistry gradients for presenting bioactive peptides

Authors:

Kipper MJ, Kleinman HK, Wang FW.

Journal:

Anal Biochem (2007): 175

Dansyl glutathione as a trapping agent for the quantitative estimation and identification of reactive metabolites

Authors:

Gan J, Harper TW, Hsueh MM, Qu Q, Humphreys WG.

Journal:

Chem Res Toxicol (2005): 896

Gold nanoparticle-based detection of genomic DNA targets on microarrays using a novel optical detection system

Authors:

Storhoff JJ, Marla SS, Bao P, Hagenow S, Mehta H, Lucas A, Garimella V, Patno T, Buckingham W, Cork W, Muller UR.

Journal:

Biosens Bioelectron (2004): 875

Fluorometric polyethyleneglycol-peptide hybrid substrates for quantitative assay of protein disulfide isomerase

Authors:

Christiansen C, St Hilaire PM, Winther JR.

Journal:

Anal Biochem (2004): 148

Assay of total homocysteine and other thiols by capillary electrophoresis and laser-induced fluorescence detection. II. Pre-analytical and analytical conditions

Authors:

Bayle C, Issac C, Salvayre R, Couderc F, Causse E.

Journal:

J Chromatogr A (2002): 255

Spectral properties

Excitation (nm)	505
Emission (nm)	524

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
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Custom size	Inquire
Technical support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Thiolite™ Green	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: GSH Standard	1 vial (62 μg)
Component D: DMSO	1 vial (400 μL)