Amplite® Fluorimetric Tyrosinase Assay Kit

Amplite® Fluorimetric Tyrosinase Assay Kit is a fluorimetric assay kit for detection of tyrosinase activity in a microplate format.

Detection Principle: Uses a fluorogenic substrate that increases fluorescence upon reaction with tyrosinase.
Signal Response: Fluorescence intensity at 440 nm correlates with tyrosinase activity.
Workflow: Simple one-step assay with a 60–120 minute incubation.
Instrument Compatibility: Fluorescence detection at Ex/Em 340/440 nm with a 420 nm cutoff using solid black microplates.

Tyrosinase dose response was measured with Amplite® Fluorimetric Tyrosinase Assay Kit in a 96-well black plate using a Gemini microplate reader (Molecular Devices). Equal volume of Tyrosinase standards and Tyrosinase Blue were added and incubated for 6 hours at 37 °C. The signal was acquired at Ex/Em = 340/440 nm (cut off at 420 nm).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11312	100 Tests	Price

Overview

Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals. Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin. The development and screening of tyrosinase inhibitors has received great attentions to melanoma related illnesses. Tyrosinase levels and activity are highly upregulated in melanoma and considered to a reliable test to monitor melanoma related illnesses. AAT Bioquest has developed Amplite® Fluorimetric Tyrosinase Assay Kit. It is a simple, one-step and reliable assay for monitoring tyrosinase activity with very high sensitivity. The assay uses a proprietary fluorogenic substrate that significantly increases its fluorescence intensity at 440 nm upon reaction with tyrosinase. The increases in fluorescence intensity at 440 nm is well correlated with tyrosinase activity. The assay kit is designed to be run with a fluorescence microplate reader.

Citations

View all 10 citations: Citation Explorer

A comprehensive review on tyrosinase inhibitors

Authors:

Zolghadri, S., Bahrami, A., Hassan Khan, M. T., Munoz-Munoz, J., Garcia-Molina, F., Garcia-Canovas, F., Saboury, A. A.

Journal:

J Enzyme Inhib Med Chem (2019): 279-309

Tyrosinase (TYR) gene sequencing and literature review reveals recurrent mutations and multiple population founder gene mutations as causative of oculocutaneous albinism (OCA) in Pakistani families

Authors:

Shakil, M., Harlalka, G. V., Ali, S., Lin, S., D'Atri, I., Hussain, S., Nasir, A., Shahzad, M. A., Ullah, M. I., Self, J. E., Baple, E. L., Crosby, A. H., Mahmood, S.

Journal:

Eye (Lond) (2019): ersion="1.0" encoding="UTF-8" ?>11311.enlEndN

Moraceae Plants with Tyrosinase Inhibitory Activity: A Review

Authors:

Burl, undefined and o, B., Clericuzio, M., Cornara, L.

Journal:

Mini Rev Med Chem (2017): 108-121

Tyrosinase inhibitors: a patent review (2011-2015)

Authors:

Ullah, S., Son, S., Yun, H. Y., Kim, D. H., Chun, P., Moon, H. R.

Journal:

Expert Opin Ther Pat (2016): 347-62

Critical review of Ayurvedic Varnya herbs and their tyrosinase inhibition effect

Authors:

Sharma, K., Joshi, N., Goyal, C.

Journal:

Anc Sci Life (2015): 18-25

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	340 nm
Emission	440 nm
Cutoff	420 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Tyrosinase Standard	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Tyrosinase Blue	1 vial
Component D: DMSO	1 vial (100 µL)