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Amplite® Fluorimetric Tyrosinase Assay Kit
Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals. Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin. The development and screening of tyrosinase inhibitors has received great attentions to melanoma related illnesses. Tyrosinase levels and activity are highly upregulated in melanoma and considered to a reliable test to monitor melanoma related illnesses. AAT Bioquest has developed Amplite® Fluorimetric Tyrosinase Assay Kit. It is a simple, one-step and reliable assay for monitoring tyrosinase activity with very high sensitivity. The assay uses a proprietary fluorogenic substrate that significantly increases its fluorescence intensity at 440 nm upon reaction with tyrosinase. The increases in fluorescence intensity at 440 nm is well correlated with tyrosinase activity. The assay kit is designed to be run with a fluorescence microplate reader.
Tyrosinase dose response was measured with Amplite® Fluorimetric Tyrosinase Assay Kit in a 96-well black plate using a Gemini microplate reader (Molecular Devices). Equal volume of Tyrosinase standards and Tyrosinase Blue were added and incubated for 6 hours at 37 °C. The signal was acquired at Ex/Em = 340/440 nm (cut off at 420 nm).
Tyrosinase dose response was measured with Amplite® Fluorimetric Tyrosinase Assay Kit in a 96-well black plate using a Gemini microplate reader (Molecular Devices). Equal volume of Tyrosinase standards and Tyrosinase Blue were added and incubated for 6 hours at 37 °C. The signal was acquired at Ex/Em = 340/440 nm (cut off at 420 nm).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11312100 Tests
Price
 
Storage, safety and handling
Intended useResearch Use Only (RUO)
Instrument settings
Fluorescence microplate reader
Excitation340 nm
Emission440 nm
Cutoff420 nm
Recommended plateSolid black
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Page updated on October 13, 2025