Amplite® Fluorimetric Xanthine Assay Kit

Amplite® Fluorimetric Xanthine Assay Kit is a fluorimetric assay kit for quantification of xanthine in biological samples.

Detection Principle: Xanthine is oxidized to uric acid by xanthine oxidase, generating H₂O₂ for fluorescent detection.
Sensitivity: Detects xanthine down to 0.4 µM in a 100 µL reaction.
Workflow: Incubates at room temperature for 30–60 minutes and supports 96- or 384-well microplates.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.

Xanthine dose response was measured with Amplite® Fluorimetric Xanthine Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13843	200 Tests	Price

Overview

Xanthine is a purine base found in most human body tissues and fluids. A number of stimulants are derived from xanthine, including caffeine, aminophylline, IBMX, paraxanthine, pentoxifylline, theobromine, and theophylline, which can stimulate heart rate, force of contraction, cardiac arrhythmias at high concentrations. Therefore, detection of Xanthine alteration in biological samples is important for disease diagnosis and therapy monitoring. Amplite® Fluorimetric Xanthine Assay Kit provides a quick and ultrasensitive method for the measurement of xanthine. It can be performed in a convenient 96-well or 384-well microtiter-plate format. Xanthine is oxidized to uric acid in the presence of xanthine oxidase to release hydrogen peroxide, which can be specifically measured with Amplite® Red by a fluorescence microplate reader. With Amplite® Fluorimetric Xanthine Assay Kit, as low as 0.4 µM xanthine was detected in a 100 µL reaction volume.

References

View all 60 references: Citation Explorer

Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation

Authors:

Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.

Journal:

J Endod (2014): 51

Effect of hypochlorite oxidation on cholinesterase-inhibition assay of acetonitrile extracts from fruits and vegetables for monitoring traces of organophosphate pesticides

Authors:

Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.

Journal:

J Toxicol Sci (2014): 71

Green synthesis of carbon dots with down- and up-conversion fluorescent properties for sensitive detection of hypochlorite with a dual-readout assay

Authors:

Yin B, Deng J, Peng X, Long Q, Zhao J, Lu Q, Chen Q, Li H, Tang H, Zhang Y, Yao S.

Journal:

Analyst (2013): 6551

Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay

Authors:

Thorn RM, Robinson GM, Reynolds DM.

Journal:

Antimicrob Agents Chemother (2013): 2216

Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite

Authors:

Setlow B, Yu J, Li YQ, Setlow P.

Journal:

Lett Appl Microbiol (2013): 259

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase (lyophilized)	1 vial
Component D: Xanthine Standard	1 vial (100 µL, 20 mM)
Component E: Xanthine Oxidase (lyophilized)	1 vial
Component F: DMSO	1 vial (100 µL)