Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Xanthine Oxidase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Xanthine Oxidase Assay Kit is a fluorimetric assay kit for quantification of xanthine oxidase activity in a microplate format.

Detection Principle: Measures H₂O₂ generated during xanthine oxidase-catalyzed oxidation of hypoxanthine or xanthine.
Sensitivity: Detects xanthine oxidase down to 0.15 mU/mL in a 100 µL reaction.
Workflow: Compatible with 96- or 384-well microplates and can be adapted to automation with no separation step.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates; absorbance detection is also supported.

Xanthine oxidase dose response was measured with Amplite® Fluorimetric Xanthine Oxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11304	200 Tests	Price

Overview

Xanthine oxidase (XO) is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. It plays an important role in the catabolism of purines. Xanthine oxidase is normally found in liver and jejunum. During severe liver damage, xanthine oxidase is released into blood, so a blood assay for XO is a way to determine if liver damage has happened. Xanthinuria is a rare genetic disorder where the lack of xanthine oxidase leads to high concentration of xanthine in blood and can cause health problems such as renal failure. The Amplite® Fluorimetric Xanthine Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of xanthine oxidase activities. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. In the assay, xanthine oxidase catalyzes the oxidation of purine bases, hypoxanthine or xanthine to uric acid and superoxide , which spontaneously degrades to hydrogen peroxide (H2O2). The kit uses our Amplite® Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Xanthine Oxidase Assay Kit, we have detected as little as 0.15 mU/mL xanthine oxidase in a 100 µL reaction volume.

Citations

View all 5 citations: Citation Explorer

Evaluation of anti-aging and antioxidant properties of a new rose variety, Ever-rose

Authors:

Han, Se Jik and Belousova, Polina and Kwon, Sangwoo and Jang, Jihui and Lee, Jun Bae and Kim, Hyunjae and You, Gayeon and Song, Jihyeon and Mok, Hyejung and Ha, Ho Su and others,

Journal:

Chemical and Biological Technologies in Agriculture (2024): 1--17

Protective effects of Cyclocarya paliurus on hyperuricemia and urate-induced inflammation

Authors:

Zhu, Li-Hua and Xu, Ying-Yin and Zhu, Li-ping and Zheng, Xian and Jiang, Cui-Hua and Liu, Jian-Jing and Zhang, Jian and Yin, Zhi-Qi

Journal:

Journal of Functional Foods (2022): 105130

Studies on Status of Oxidative Stress related Molecules and Enzymes in Obese with and without Diabetes in the Northern region of India

Authors:

Singh, Sukhpal and Mahajan, Amita and Kaur, Jaspreet

Journal:

Research Journal of Pharmacy and Technology (2020): 801--809

Xanthine oxidoreductase regulates macrophage IL1$\beta$ secretion upon NLRP3 inflammasome activation

Authors:

Ives, Annette and Nomura, Johji and Martinon, Fabio and Roger, Thierry and LeRoy, Didier and Miner, Jeffrey N and Simon, Gregoire and Busso, Nathalie and So, Alexander

Journal:

Nature communications (2015): 1--11

Xanthine oxidoreductase regulates macrophage IL1β secretion upon NLRP3 inflammasome activation

Authors:

Ives, Annette and Nomura, Johji and Martinon, Fabio and Roger, Thierry and LeRoy, Didier and Miner, Jeffrey N and Simon, Gregoire and Busso, Nathalie and So, Alex and er, undefined

Journal:

Nature communications (2015)

References

View all 52 references: Citation Explorer

Phenotyping of N-acetyltransferase type 2 and xanthine oxidase with caffeine: when should urine samples be collected

Authors:

Jetter A, Kinzig M, Rodamer M, Tomalik-Scharte D, Sorgel F, Fuhr U.

Journal:

Eur J Clin Pharmacol (2009): 411

Measurement of xanthine oxidase inhibition activity of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method

Authors:

Ozyurek M, Bektasoglu B, Guclu K, Apak R.

Journal:

Anal Chim Acta (2009): 42

In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb

Authors:

Umamaheswari M, Asokkumar K, Sivashanmugam AT, Remyaraju A, Subhadradevi V, Ravi TK.

Journal:

J Ethnopharmacol (2009): 646

Increased xanthine oxidase in the skin of preeclamptic women

Authors:

Bainbridge SA, Deng JS, Roberts JM.

Journal:

Reprod Sci (2009): 468

Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice

Authors:

Chatterjee S, Ehrenshaft M, Bhattacharjee S, Deterding LJ, Bonini MG, Corbett J, Kadiiska MB, Tomer KB, Mason RP.

Journal:

Free Radic Biol Med (2009): 454

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540nm
Emission	590nm
Cutoff	570nm
Recommended plate	Solid black

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Page updated on October 28, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase	1 vial (lyophilized)
Component D: Xanthine	1 vial (100 µL, 100 X)
Component E: Xanthine Oxidase Standard	1 vial (200 mU, lyophilized)
Component F: DMSO	1 vial (200 µL)