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Amplite® Fluorimetric Zinc Ion Quantitation Kit
Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring are highly desirable in research and drug discovery. AAT Bioquest's Amplite® Fluorimetric Zinc Quantitation Kit provides a simple method for detecting zinc concentration in biological samples using our proprietary Metaltel; Zn 520, in which Zinc binds to the probe with enhanced fluorescence. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+-specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).
Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Ion Quantitation Kit.
Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Ion Quantitation Kit.
CatalogSize
Price
Quantity
19000200 Tests
Price
 
Spectral properties

Excitation (nm)493
Emission (nm)516
Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
UNSPSC41116134
Instrument settings

Fluorescence microplate reader
Excitation485 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on September 25, 2025