Amplite® Fluorimetric Zinc Ion Quantitation Kit

Amplite® Fluorimetric Zinc Ion Quantitation Kit is a fluorimetric assay kit for detection of zinc ions in biological samples such as serum, plasma, and urine.

Detection Principle: Metal Fluor™ Zn 520 exhibits enhanced fluorescence upon binding Zn²⁺.
Sensitivity: Detects Zn²⁺ down to 0.2 µM (13 ng/mL).
Specificity: Shows high Zn²⁺ selectivity with little response to Ca²⁺, Mg²⁺, Mn²⁺, and Cu²⁺.
Signal Advantage: Provides >200–300-fold fluorescence enhancement in response to Zn²⁺.
Instrument Compatibility: Fluorescence detection at Ex/Em 485/525 nm using solid black microplates.

Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Ion Quantitation Kit.

Protocol

SDS

Catalog	Size	Price	Quantity
19000	200 Tests	Price

Overview

Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring are highly desirable in research and drug discovery. AAT Bioquest's Amplite® Fluorimetric Zinc Quantitation Kit provides a simple method for detecting zinc concentration in biological samples using our proprietary Metaltel; Zn 520, in which Zinc binds to the probe with enhanced fluorescence. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+-specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).

Citations

View all 5 citations: Citation Explorer

Simple Zinc Metallic Particle Doping Transforms Ceramic Bone Cement Therapeutic Performance

Authors:

Zhou, Juncen and Li, Bing and Zhou, Jiayi and Abasolo, Sai Aishwarya and Akhter, Firoz and Akhter, Asma and Xing, Malcolm and Cheng, Ke and Zhu, Donghui

Journal:

Advanced Functional Materials (2025): e14417

Yeast-Derived Manganese and Zinc Metal--Organic Framework Composite as a Vaccine Adjuvant for Enhanced Humoral and Cellular Immune Responses

Authors:

Zheng, Lanlan and Wang, Zi and Liu, Hang and Wang, Nianxiang and Liu, Junjun and Ma, Mengyao and Jia, Xinhao and Qian, Mengwei and Liu, Yidan and Li, Muzi and others,

Journal:

ACS nano (2025)

ZIP1+ fibroblasts protect lung cancer against chemotherapy via connexin-43 mediated intercellular Zn2+ transfer

Authors:

Ni, Chen and Lou, Xiaohan and Yao, Xiaohan and Wang, Linlin and Wan, Jiajia and Duan, Xixi and Liang, Jialu and Zhang, Kaili and Yang, Yuanyuan and Zhang, Li and others,

Journal:

Nature communications (2022): 1--20

Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision

Authors:

Chakraborty, Tapas and Mohapatra, Sipra and Tobayama, Megumi and Ohta, Kayoko and Ryu, Yong-Woon and Kazeto, Yukinori and Ohta, Kohei and Zhou, Linyan and Nagahama, Yoshitaka and Matsubara, Takahiro

Journal:

Scientific reports (2017): 1--12

After oxidation, zinc nanoparticles lose their ability to enhance responses to odorants

Authors:

Hagerty, Samantha and Daniels, Yasmine and Singletary, Melissa and Pustovyy, Oleg and Globa, Ludmila and MacCrehan, William A and Muramoto, Shin and Stan, Gheorghe and Lau, June W and Morrison, Edward E and others, undefined

Journal:

BioMetals (2016): 1--14

References

View all 46 references: Citation Explorer

Reaction of metal-binding ligands with the zinc proteome: zinc sensors and N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine

Authors:

Meeusen JW, Nowakowski A, Petering DH.

Journal:

Inorg Chem (2012): 3625

EAAC1 gene deletion alters zinc homeostasis and enhances cortical neuronal injury after transient cerebral ischemia in mice

Authors:

Jang BG, Won SJ, Kim JH, Choi BY, Lee MW, Sohn M, Song HK, Suh SW.

Journal:

J Trace Elem Med Biol (2012): 85

TSQ (6-methoxy-8-p-toluenesulfonamido-quinoline), a common fluorescent sensor for cellular zinc, images zinc proteins

Authors:

Meeusen JW, Tomasiewicz H, Nowakowski A, Petering DH.

Journal:

Inorg Chem (2011): 7563

Dependence of the histofluorescently reactive zinc pool on zinc transporter-3 in the normal brain

Authors:

Lee JY, Kim JS, Byun HR, Palmiter RD, Koh JY.

Journal:

Brain Res (2011): 12

Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution

Authors:

Nowakowski AB, Petering DH.

Journal:

Inorg Chem (2011): 10124

Spectral properties

Excitation (nm)	493
Emission (nm)	516

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	485 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Metal Fluor™ Zn 520	1 vial (50 µL)
Component B: Assay Buffer	1 bottle (15 mL)
Component C: ZnCl2 Standard	1 vial (100 mM,100 µL)