Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring are highly desirable in research and drug discovery. AAT Bioquest's Amplite® Fluorimetric Zinc Quantitation Kit provides a simple method for detecting zinc concentration in biological samples using our proprietary Metaltel; Zn 520, in which Zinc binds to the probe with enhanced fluorescence. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+-specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).
Zheng, Lanlan and Wang, Zi and Liu, Hang and Wang, Nianxiang and Liu, Junjun and Ma, Mengyao and Jia, Xinhao and Qian, Mengwei and Liu, Yidan and Li, Muzi and others,
Ni, Chen and Lou, Xiaohan and Yao, Xiaohan and Wang, Linlin and Wan, Jiajia and Duan, Xixi and Liang, Jialu and Zhang, Kaili and Yang, Yuanyuan and Zhang, Li and others,
Journal:
Nature communications (2022): 1--20
Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
Authors:
Chakraborty, Tapas and Mohapatra, Sipra and Tobayama, Megumi and Ohta, Kayoko and Ryu, Yong-Woon and Kazeto, Yukinori and Ohta, Kohei and Zhou, Linyan and Nagahama, Yoshitaka and Matsubara, Takahiro
Hagerty, Samantha and Daniels, Yasmine and Singletary, Melissa and Pustovyy, Oleg and Globa, Ludmila and MacCrehan, William A and Muramoto, Shin and Stan, Gheorghe and Lau, June W and Morrison, Edward E and others, undefined