Name: Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
Brand: AAT Bioquest
SKU: 12530
Availability: InStock

Amplite® Gaussia Luciferase Reporter Gene Assay Kit

Maximized Luminescence

Amplite® Gaussia Luciferase Reporter Gene Assay Kit is a chemiluminescent assay kit for quantification of Gaussia luciferase activity in cell culture medium.

Detection Principle: Gaussia luciferase catalyzes coelenterazine oxidation to generate luminescence.
Signal Advantage: Produces a glow-type signal with an approximately 1-hour half-life for consistent plate-to-plate measurement.
Workflow: Compatible with HTS liquid handling and 96- or 384-well microplates.
Applications: Measures secreted Gaussia luciferase directly in standard cell growth media.

Sectreated Gaussia Luciferase culture medium was measured with Amplite® Gaussia Luciferase Reporter Gene Assay Kit (blue line) and a commercially available Gaussia Luciferase Assay Kit (red line) in a white 96-well plate with a NOVOstar plate reader (BMG Labtech).

Protocol

Catalog	Size	Price
12530	1 plate	Price
12531	10 Plates	Price
12532	100 plates	Price

Overview

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Gaussia luciferase since these reporters are smaller and do not require the presence of ATP. The bioluminescent enzyme derived from the marine copepod Gaussia prince is efficiently secreted from mammalian cells upon expression. Gaussia luciferase is a 20kDa protein which catalyzes coelenterazine oxidation by oxygen to produce light. Our Amplite® Gaussia Luciferase Reporter Gene Assay Kit uses a proprietary luminogenic formulation to quantify luciferase activity in cell medium. Our formulation generates a luminescent product that gives strong luminescence upon interaction with Gaussia luciferase. The kit provides all the essential components that are compatible with HTS liquid handling instruments. The kit has high sensitivity, and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible standard cell growth media.

Citations

View all 2 citations: Citation Explorer

Use of Tox21 screening data to profile PFAS bioactivities on nuclear receptors, cellular stress pathways, and cytochrome p450 enzymes

Authors:

Ooka, Masato and Sakamuru, Srilatha and Zhao, Jinghua and Qu, Yanyan and Fang, Yuhong and Tao, Dingyin and Huang, Ruili and Ferguson, Stephen and Reif, David and Simeonov, Anton and others,

Journal:

Journal of Hazardous Materials (2024): 134642

Identification and profiling of environmental chemicals that inhibit the TGFβ/SMAD signaling pathway

Authors:

Wei, Zhengxi and Sakamuru, Srilatha and Zhang, Li and Zhao, Jinghua and Huang, Ruili and Kleinstreuer, Nicole C and Chen, Yanling and Shu, Yan and Knudsen, Thomas B and Xia, Menghang

Journal:

Chemical research in toxicology (2019)

References

View all 18 references: Citation Explorer

Secreted Gaussia luciferase as a biomarker for monitoring tumor progression and treatment response of systemic metastases

Authors:

Chung E, Yamashita H, Au P, Tannous BA, Fukumura D, Jain RK.

Journal:

PLoS One (2009): e8316

Cell-free production of Gaussia princeps luciferase--antibody fragment bioconjugates for ex vivo detection of tumor cells

Authors:

Patel KG, Ng PP, Kuo CC, Levy S, Levy R, Swartz JR.

Journal:

Biochem Biophys Res Commun (2009): 971

Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase

Authors:

Santos EB, Yeh R, Lee J, Nikhamin Y, Punzalan B, La Perle K, Larson SM, Sadelain M, Brentjens RJ.

Journal:

Nat Med (2009): 338

A multifunctional, synthetic Gaussia princeps luciferase reporter for live imaging of Candida albicans infections

Authors:

Enjalbert B, Rachini A, Vediyappan G, Pietrella D, Spaccapelo R, Vecchiarelli A, Brown AJ, d'Enfert C.

Journal:

Infect Immun (2009): 4847

Split Gaussia luciferase-based bioluminescence template for tracing protein dynamics in living cells

Authors:

Kim SB, Sato M, Tao H.

Journal:

Anal Chem (2009): 67

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 26, 2026

Component A: Luciferase Substrate (Light-sensitive)	1 vial
Component B: Reaction Buffer	1 vial (50 µL)
Component C: Assay Buffer	1 bottle (5 mL)

Component A: Luciferase Substrate	1 vial
Component B: Reaction Buffer	1 vial (0.5 mL)
Component C: Assay Buffer	1 bottle (50 mL)

Component A: Luciferase Substrate	2 vials
Component B: Reaction Buffer	1 bottle (5 mL)
Component C: Assay Buffer	1 bottle (500 mL)