Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner. Several different ligand binding sites have been identified for HSA. Among them, Site I has been identified as one of major drug binding sites. Amplite®™ Human Serum Albumin (HSA) Site I Binding Assay Kit is a fluorescence-based high throughput assay to determine the small molecule binding towards HSA. This assay is based on a novel fluorescent probe, HSA Blue™ S1. It has been characterized to bind to the site 1 of HSA with unique spectroscopic and binding properties. HSA Blue™ S1 displays a large fluorescence intensity difference between the protein-bound and protein-unbound state. The competition of small molecules for HSA binding in the presence of HSA Blue™ S1 results in low fluorescence intensities. This assay can be used as a high throughput screen tool to determine total binding to HSA at Site I.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25400	200 Tests	Price

References

View all 50 references: Citation Explorer

Anionic versus neutral Pt(II) complexes: The relevance of the charge for human serum albumin binding.

Authors:

Ricciardi, Loredana and Guzzi, Rita and Rizzuti, Bruno and Ionescu, Andreea and Aiello, Iolinda and Ghedini, Mauro and La Deda, Massimo

Journal:

Journal of inorganic biochemistry (2020): 111024

Human Serum Albumin Binding in a Vial: A Novel UV-pH Titration Method To Assist Drug Design.

Authors:

Dargó, Gergő and Bajusz, Dávid and Simon, Kristóf and Müller, Judit and Balogh, György T

Journal:

Journal of medicinal chemistry (2020): 1763-1774

Anticancer activity, calf thymus DNA and human serum albumin binding properties of Farnesiferol C from Ferula pseudalliacea.

Authors:

Tanzadehpanah, Hamid and Mahaki, Hanie and Samadi, Pouria and Karimi, Jamshid and Moghadam, Neda Hosseinpour and Salehzadeh, Sadegh and Dastan, Dara and Saidijam, Massoud

Journal:

Journal of biomolecular structure & dynamics (2019): 2789-2800

An integrated quantitative structure and mechanism of action-activity relationship model of human serum albumin binding.

Authors:

Serra, Angela and Önlü, Serli and Coretto, Pietro and Greco, Dario

Journal:

Journal of cheminformatics (2019): 38

The displacement study of 99m Tc-DTPA-Human serum albumin binding in presence of furosemide and metformin by using equilibrium dialysis and molecular docking.

Authors:

Chemlal, Laila and Akachar, Jihane and Makram, Sanaa and Zoubir, Brahim and Cherrah, Yahia and Eljaoudi, Rachid and Ibrahimi, Azeddine and Faouzi, Mly A

Journal:

IUBMB life (2019): 2003-2009

Spectral properties

Excitation (nm)	334
Emission (nm)	511

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	365 nm
Emission	480 nm
Cutoff	435 nm
Recommended plate	Solid black
Instrument specification(s)	Top read mode

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Page updated on September 30, 2025

Component A: HSA Blue™ S1	1 vial
Component B: HSA Assay Buffer	1 bottle (30 mL)
Component C: Human Serum Albumin solution (HSA)	1 vial (500 µL)
Component D: DMSO	1 vial (100 µL)