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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner. Several different ligand binding sites have been identified for HSA. Among them, Site I has been identified as one of major drug binding sites. Amplite®™ Human Serum Albumin (HSA) Site I Binding Assay Kit is a fluorescence-based high throughput assay to determine the small molecule binding towards HSA. This assay is based on a novel fluorescent probe, HSA Blue™ S1. It has been characterized to bind to the site 1 of HSA with unique spectroscopic and binding properties. HSA Blue™ S1 displays a large fluorescence intensity difference between the protein-bound and protein-unbound state. The competition of small molecules for HSA binding in the presence of HSA Blue™ S1 results in low fluorescence intensities. This assay can be used as a high throughput screen tool to determine total binding to HSA at Site I.
Response of Warfarin (Site-1 drug) and Ibuprofen (Site-2 drug) was measured using Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit. The response was acquired using Spectramax Gemini XS (Molecular devices) with Ex/Em = 365/480 nm with cutoff = 435 nm.
Response of Warfarin (Site-1 drug) and Ibuprofen (Site-2 drug) was measured using Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit. The response was acquired using Spectramax Gemini XS (Molecular devices) with Ex/Em = 365/480 nm with cutoff = 435 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
25400200 Tests
Price
 
Spectral properties
Excitation (nm)334
Emission (nm)511
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation365 nm
Emission480 nm
Cutoff435 nm
Recommended plateSolid black
Instrument specification(s)Top read mode
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 26, 2025