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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner. Several different ligand binding sites have been identified for HSA. Among them, Site II has been identified as one of major drug binding sites. Amplite®™ Human Serum Albumin (HSA) Site II Binding Assay Kit is a fluorescence-based high throughput assay to determine the small molecule binding towards HSA. This assay is based on a novel fluorescent probe, HSA Blue™ S2. It has been characterized to bind to the site 2 of HSA with unique spectroscopic and binding properties. HSA Blue™ S2 displays a large fluorescence intensity difference between the protein-bound and protein-unbound state. The competition of small molecules for HSA binding in the presence of HSA Blue™ S2 results in low fluorescence intensities. This assay can be used as a high throughput screen tool to determine total binding to HSA at Site II.
Response of Warfarin (Site-1 drug) and Ibuprofen (Site-2 drug) was measured using Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit. The response was acquired using Spectra Max Gemini XS (Molecular devices) with Ex/Em = 365/480 nm with cutoff = 435 nm.
Response of Warfarin (Site-1 drug) and Ibuprofen (Site-2 drug) was measured using Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit. The response was acquired using Spectra Max Gemini XS (Molecular devices) with Ex/Em = 365/480 nm with cutoff = 435 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
25402200 Tests
Price
 
Spectral properties
Excitation (nm)337
Emission (nm)495
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 13, 2025